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BacDive ID 130968
Type strain
Strain Designation I49
Culture col. no. DSM 32035 I49
NCBI tax ID(s) 1598
Links
version 10 (current version)

General

@ref: 22260

BacDive-ID: 130968

DSM-Number: 32035

keywords: genome sequence, 16S sequence, microaerophile, mesophilic

description: Limosilactobacillus reuteri I49 is a microaerophile, mesophilic prokaryote that was isolated from C57BL/6J wildtype mouse.

NCBI tax id

  • NCBI tax id: 1598
  • Matching level: species

strain history

  • @ref: 22260
  • history: <- B. Stecher, Max von Pettenkofer-Inst., Munich; I49

doi: 10.13145/bacdive130968.20251217.10

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: kingdom/bacillati
  • keyword: phylum/bacillota
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Limosilactobacillus
  • species: Limosilactobacillus reuteri
  • full scientific name: Limosilactobacillus reuteri (Kandler et al. 1982) Zheng et al. 2020
  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus reuteri

@ref: 22260

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Limosilactobacillus

species: Limosilactobacillus reuteri

full scientific name: Limosilactobacillus reuteri (Kandler et al. 1982) Zheng et al. 2020

strain designation: I49

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram stain
125438no90
12543892.582positive

colony morphology

  • @ref: 22260
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

  • @ref: 22260
  • name: MRS MEDIUM (DSMZ Medium 11)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/11
  • composition: Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

culture temp

  • @ref: 22260
  • growth: positive
  • type: growth
  • temperature: 37

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
22260microaerophile
125439microaerophile98

Isolation, sampling and environmental information

isolation

  • @ref: 22260
  • sample type: C57BL/6J wildtype mouse
  • geographic location: Zurich
  • country: Switzerland
  • origin.country: CHE
  • continent: Europe

isolation source categories

  • Cat1: #Host
  • Cat2: #Mammals
  • Cat3: #Muridae (Mouse/Rat)

taxonmaps

  • @ref: 69479
  • File name: preview.99_581.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_369;97_416;98_475;99_581&stattab=map
  • Last taxonomy: Limosilactobacillus reuteri
  • 16S sequence: KR364776
  • Sequence Identity:
  • Total samples: 169334
  • soil counts: 4867
  • aquatic counts: 7596
  • animal counts: 154945
  • plant counts: 1926

Interaction and safety

risk assessment

  • @ref: 22260
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

@refdescriptionassembly levelINSDC accessionIMG accessionNCBI tax IDscoreBV-BRC accession
66792ASM168868v2 assembly for Limosilactobacillus reuteri I49completeGCA_0016886852718217763159897.31
66792ASM1669704v1 assembly for Limosilactobacillus reuteri I49chromosomeGCA_0166970458003878414159887.031598.1406
66792ASM222165v1 assembly for Limosilactobacillus reuteri I49contigGCA_0022216552902505161159877.681598.349

16S sequences

  • @ref: 22260
  • description: Limosilactobacillus reuteri strain I49 16S ribosomal RNA gene, partial sequence
  • accession: KR364776
  • length: 1560
  • database: nuccore
  • NCBI tax ID: 1598

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingyes92.582no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no83.879yes
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no92.803yes
125438spore-formingspore-formingAbility to form endo- or exosporesno89.656no
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno97.375yes
125438motile2+flagellatedAbility to perform flagellated movementno90no
125439BacteriaNetspore_formationAbility to form endo- or exosporesno86
125439BacteriaNetmotilityAbility to perform movementno82
125439BacteriaNetgram_stainReaction to gram-stainingpositive88.4
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthmicroaerophile98

Literature

@ref: 22260

culture collection no.: DSM 32035

straininfo link

  • @ref: 126262
  • straininfo: 402237

literature

titleauthorsjournalyearmeshPubmed-IDDOItopic
The human intestinal bacterium Eggerthella lenta influences gut metabolomes in gnotobiotic miceViehof A, Haange S, Streidl T, Schubert K, Engelmann B, Haller D, Rolle-Kampczyk U, von Bergen M, Clavel T.Microbiome Res Rep2024Proteomics, Metabolomics, Gut Microbiota, Gut-liver Axis, Eggerthella Lenta, Oligo-mouse-microbiota
Nutritional and host environments determine community ecology and keystone species in a synthetic gut bacterial community.Weiss AS, Niedermeier LS, von Strempel A, Burrichter AG, Ring D, Meng C, Kleigrewe K, Lincetto C, Hubner J, Stecher B.Nat Commun2023Microbiota, Gastrointestinal Microbiome3755333610.1038/s41467-023-40372-0
Bacteriophages targeting protective commensals impair resistance against Salmonella Typhimurium infection in gnotobiotic mice.von Strempel A, Weiss AS, Wittmann J, Salvado Silva M, Ring D, Wortmann E, Clavel T, Debarbieux L, Kleigrewe K, Stecher B.PLoS Pathog2023Bacteriophages, Salmonella Infections, Microbiota, Gastrointestinal Microbiome3760355810.1371/journal.ppat.1011600
In vitro and in vivo selection and cost of bacteriophage resistance on natural Escherichia coli.Cardoso LL, Gaissmaier MS, von Strempel A, Keys T, Matchado MS, Salvado Silva M, Ring D, Slack E, Stecher B.Microlife2025Mutations, Diarrhea, Intestinal Infection, Phage Therapy, Eskape, Phage Efficacy, Within Host Evolution4083784210.1093/femsml/uqaf017Pathogenicity

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22260Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-32035Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 32035)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg OvermannPredicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets10.1101/2024.08.12.607695
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardydeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1https://github.com/GenomeNet/deepG
126262A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. ReimerStrainInfo—the central database for linked microbial strain identifiers10.1093/database/baaf059