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BacDive ID 140257
Type strain
Strain Designation CDW1, P6 Po
Culture col. no. DSM 101753 LMG 29236 CDW1 P6 Po
NCBI tax ID(s) 1755100
Links
version 10 (current version)

General

@ref: 42864

BacDive-ID: 140257

DSM-Number: 101753

keywords: 16S sequence, mesophilic

description: Fusobacterium gastrosuis CDW1 is a mesophilic prokaryote that was isolated from pars oesophagea of the stomach of a 6-months old pig.

NCBI tax id

  • NCBI tax id: 1755100
  • Matching level: species

strain history

  • @ref: 42864
  • history: <- C. De Witte, Univ. Ghent, Belgium; CDW1

doi: 10.13145/bacdive140257.20251217.10

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: kingdom/fusobacteriati
  • keyword: phylum/fusobacteriota
  • phylum: Fusobacteriota
  • class: Fusobacteriia
  • order: Fusobacteriales
  • family: Fusobacteriaceae
  • genus: Fusobacterium
  • species: Fusobacterium gastrosuis
  • full scientific name: Fusobacterium gastrosuis De Witte et al. 2017

@ref: 42864

domain: Bacteria

phylum: Fusobacteria

class: Fusobacteriia

order: Clostridiales

family: Fusobacteriaceae

genus: Fusobacterium

species: Fusobacterium gastrosuis

full scientific name: Fusobacterium gastrosuis De Witte et al. 2017

strain designation: CDW1, P6 Po

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
42864COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
42864PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water

culture temp

  • @ref: 42864
  • growth: positive
  • type: growth
  • temperature: 37

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838017632nitrate-reduction
6838016634raffinose-fermentation
6838016024D-mannose-fermentation
6838029016arginine-hydrolysis
6838027897tryptophan+energy source
6838029985L-glutamate-degradation
6838016199urea-hydrolysis

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
68380glycin arylamidase-
68380histidine arylamidase-
68380glutamyl-glutamate arylamidase-
68380serine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380tryptophan deaminase+4.1.99.1
68380glutamate decarboxylase-4.1.1.15
68380alpha-fucosidase-3.2.1.51
68380alpha-arabinosidase-3.2.1.55
68380beta-glucuronidase-3.2.1.31
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380urease-3.5.1.5
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase+3.1.3.1
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380beta-glucosidase-3.2.1.21
68380arginine dihydrolase-3.5.3.6

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
42864---------------++-+----------
42864---------------++-+----------

Isolation, sampling and environmental information

isolation

  • @ref: 42864
  • sample type: pars oesophagea of the stomach of a 6-months old pig
  • geographic location: Ghent
  • country: Belgium
  • origin.country: BEL
  • continent: Europe
  • latitude: 51.0543
  • longitude: 3.7174

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Suidae (Pig,Swine)
#Host Body-Site#Gastrointestinal tract#Stomach

Sequence information

16S sequences

  • @ref: 42864
  • description: Fusobacterium gastrosuis partial 16S rRNA gene, type strain CDW1T, isolate P6 Po
  • accession: LN906797
  • length: 1512
  • database: nuccore
  • NCBI tax ID: 1755100

GC content

  • @ref: 42864
  • GC-content: 28.2
  • method: high performance liquid chromatography (HPLC)

Literature

@ref: 42864

culture collection no.: DSM 101753, LMG 29236

straininfo link

  • @ref: 126262
  • straininfo: 402731

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny27816261Detection, isolation and characterization of Fusobacterium gastrosuis sp. nov. colonizing the stomach of pigs.De Witte C, Flahou B, Ducatelle R, Smet A, De Bruyne E, Cnockaert M, Taminiau B, Daube G, Vandamme P, Haesebrouck FSyst Appl Microbiol10.1016/j.syapm.2016.10.0012016Anaerobiosis, Animals, Bacterial Typing Techniques, Base Composition, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Fusobacterium/*classification/genetics/*isolation & purification, Fusobacterium Infections/microbiology/*veterinary, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Stomach/*microbiology, SwineTranscriptome
39135697Genetic manipulations of nonmodel gut microbes.Jin WB, Guo CJ.Imeta10.1002/imt2.2162024Human Gut Microbiota, Genetic Manipulation Strategies, Nonmodel Gut Bacteroidia, Nonmodel Gut Clostridia

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
42864Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-101753Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 101753)
68380Automatically annotated from API rID32A
126262A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. ReimerStrainInfo—the central database for linked microbial strain identifiers10.1093/database/baaf059