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BacDive ID 24289
Type strain
Strain Designation Br
Culture col. no. DSM 27245 ICMP 19912 Br
NCBI tax ID(s) 1380763
Links
version 10 (current version)

General

@ref: 20713

BacDive-ID: 24289

DSM-Number: 27245

keywords: genome sequence, 16S sequence, psychrophilic

description: Paenibacillus darwinianus Br is a psychrophilic prokaryote that was isolated from gamma-irradiated soil.

NCBI tax id

  • NCBI tax id: 1380763
  • Matching level: species

strain history

  • @ref: 20713
  • history: <- M. Dsouza, University of Auckland, New Zealand; strain Br

doi: 10.13145/bacdive24289.20251217.10

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: kingdom/bacillati
  • keyword: phylum/bacillota
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Paenibacillus
  • species: Paenibacillus darwinianus
  • full scientific name: Paenibacillus darwinianus Dsouza et al. 2014

@ref: 20713

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Paenibacillaceae

genus: Paenibacillus

species: Paenibacillus darwinianus

full scientific name: Paenibacillus darwinianus Dsouza et al. 2014

strain designation: Br

type strain: yes

Morphology

cell morphology

  • @ref: 125439
  • motility: yes
  • confidence: 90.9

colony morphology

  • @ref: 20713
  • incubation period: >14 days

Culture and growth conditions

culture medium

  • @ref: 20713
  • name: PYGV AGAR (DSMZ Medium 621)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/621
  • composition: Name: PYGV AGAR (DSMZ Medium 621) Composition: Agar 15.0 g/l MgSO4 x 7 H2O 0.625264 g/l Glucose 0.25 g/l Peptone 0.25 g/l Yeast extract 0.25 g/l Nitrilotriacetic acid 0.210526 g/l CaCl2 x 2 H2O 0.0703158 g/l FeSO4 x 7 H2O 0.00208422 g/l Na2MoO4 x 2 H2O 0.000266736 g/l Pyridoxine hydrochloride 0.0001 g/l p-Aminobenzoic acid 5e-05 g/l D-Calcium pantothenate 5e-05 g/l Nicotinamide 5e-05 g/l Thiamine-HCl x 2 H2O 5e-05 g/l Riboflavine 5e-05 g/l Folic acid 2e-05 g/l Biotin 2e-05 g/l Vitamin B12 1e-06 g/l Metall salt sol. 44 Distilled water

culture temp

  • @ref: 20713
  • growth: positive
  • type: growth
  • temperature: 20

Physiology and metabolism

oxygen tolerance

  • @ref: 125439
  • oxygen tolerance: aerobe
  • confidence: 96.4

spore formation

  • @ref: 125439
  • spore formation: yes
  • confidence: 93.5

Isolation, sampling and environmental information

isolation

  • @ref: 20713
  • sample type: gamma-irradiated soil
  • geographic location: Britannia drift, Lake Wellman Region in the the south-eastern Darwin Mountains (79° 55' 16.2'' S 156° 55' 30.7'' E)
  • continent: Australia and Oceania
  • latitude: -54.665
  • longitude: -69.664

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_2752.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15358;96_1464;97_1746;98_2135;99_2752&stattab=map
  • Last taxonomy: Paenibacillus darwinianus subclade
  • 16S sequence: KF264455
  • Sequence Identity:
  • Total samples: 635
  • soil counts: 168
  • aquatic counts: 79
  • animal counts: 357
  • plant counts: 31

Interaction and safety

risk assessment

  • @ref: 20713
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

  • @ref: 66792
  • description: Brdraft1 assembly for Paenibacillus darwinianus Br
  • assembly level: scaffold
  • INSDC accession: GCA_000598065
  • BV-BRC accession: 1380763.3
  • IMG accession: 2609460045
  • NCBI tax ID: 1380763
  • score: 8.55

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20713Paenibacillus darwinianus strain Br 16S ribosomal RNA gene, partial sequenceKF2644551412nuccore1380763
124043Paenibacillus darwinianus strain MB 16S ribosomal RNA gene, partial sequence.KF2644561412nuccore1380763
124043Paenibacillus darwinianus strain MB 16S ribosomal RNA gene, partial sequence.KF2644571409nuccore1380763

GC content

  • @ref: 20713
  • GC-content: 55.6
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingyes71.566no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no92.247no
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes73.295no
125438spore-formingspore-formingAbility to form endo- or exosporesyes84.425no
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno76.02no
125438motile2+flagellatedAbility to perform flagellated movementyes79.743no
125439BacteriaNetspore_formationAbility to form endo- or exosporesyes93.5
125439BacteriaNetmotilityAbility to perform movementyes90.9
125439BacteriaNetgram_stainReaction to gram-stainingvariable81.3
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthaerobe96.4

Literature

@ref: 20713

culture collection no.: DSM 27245, ICMP 19912

straininfo link

  • @ref: 126262
  • straininfo: 403781

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny24449790Paenibacillus darwinianus sp. nov., isolated from gamma-irradiated Antarctic soil.Dsouza M, Taylor MW, Ryan J, MacKenzie A, Lagutin K, Anderson RF, Turner SJ, Aislabie JInt J Syst Evol Microbiol10.1099/ijs.0.056697-02014Antarctic Regions, Bacterial Typing Techniques, Base Composition, Fatty Acids/chemistry, Molecular Sequence Data, Paenibacillus/*classification/genetics/isolation & purification, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryGenetics
25285990Genome-based comparative analyses of Antarctic and temperate species of Paenibacillus.Dsouza M, Taylor MW, Turner SJ, Aislabie J.PLoS One10.1371/journal.pone.01080092014genetics, genetics

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
20713Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-27245Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 27245)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
124043Isabel Schober, Julia KoblitzData extracted from sequence databases, automatically matched based on designation and taxonomy
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg OvermannPredicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets10.1101/2024.08.12.607695
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardydeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1https://github.com/GenomeNet/deepG
126262A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. ReimerStrainInfo—the central database for linked microbial strain identifiers10.1093/database/baaf059