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BacDive ID 4285
Type strain
Culture col. no. DSM 15116 SG 3301 JCM 17835
NCBI tax ID(s) 168379
Links
version 10 (current version)

General

@ref: 5718

BacDive-ID: 4285

DSM-Number: 15116

keywords: genome sequence, 16S sequence, anaerobe, mesophilic

description: Halorhodospira neutriphila DSM 15116 is an anaerobe, mesophilic prokaryote that was isolated from microbial mat in marine saltern.

NCBI tax id

  • NCBI tax id: 168379
  • Matching level: species

strain history

@refhistory
5718<- P. Caumette; SG3301 <- R. Matheron
67770A. Hirschler-Réa SG 3301.

doi: 10.13145/bacdive4285.20251217.10

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: kingdom/pseudomonadati
  • keyword: phylum/pseudomonadota
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Chromatiales
  • family: Halorhodospiraceae
  • genus: Halorhodospira
  • species: Halorhodospira neutriphila
  • full scientific name: Halorhodospira neutriphila Hirschler-Réa et al. 2003

@ref: 5718

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Chromatiales

family: Ectothiorhodospiraceae

genus: Halorhodospira

species: Halorhodospira neutriphila

full scientific name: Halorhodospira neutriphila Hirschler-Réa et al. 2003

type strain: yes

Morphology

cell morphology

@refgram stainconfidence
125438negative98.75
125439negative91.3

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5718HALORHODOSPIRA MEDIUM (DSMZ Medium 999)yeshttps://mediadive.dsmz.de/medium/999Name: HALORHODOSPIRA MEDIUM (DSMZ Medium 999) Composition: NaCl 100.0 g/l NaHCO3 2.0 g/l MgCl2 x 6 H2O 2.0 g/l KH2PO4 1.0 g/l MgSO4 x 7 H2O 1.0 g/l Na2S x 9 H2O 0.75 g/l NH4Cl 0.5 g/l CaCl2 x 2 H2O 0.05 g/l FeSO4 x 7 H2O 0.0011 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.00019 g/l Pyridoxamine 5e-05 g/l MnCl2 x 2 H2O 5e-05 g/l ZnCl2 4.2e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Vitamine B12 2e-05 g/l Nicotinate 2e-05 g/l Na2MoO4 x 2 H2O 1.8e-05 g/l p-Aminobenzoate 1e-05 g/l Thiamine 1e-05 g/l Pantothenate 5e-06 g/l Biotine 2e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water
5718CHROMATIUM SALEXIGENS MEDIUM (DSMZ Medium 421)yeshttps://mediadive.dsmz.de/medium/421Name: CHROMATIUM SALEXIGENS MEDIUM (DSMZ Medium 421) Composition: NaCl 100.0 g/l MgCl2 x 6 H2O 3.0 g/l NaHCO3 1.5 g/l Na2S x 9 H2O 0.592592 g/l MgSO4 x 7 H2O 0.500001 g/l Na acetate 0.5 g/l Sodium Thiosulfate 0.5 g/l Ammonium chloride 0.349999 g/l KH2PO4 0.349999 g/l KCl 0.349999 g/l CaCl2 x 2 H2O 0.25 g/l Dextrose 0.25 g/l Pyruvic acid sodium salt 0.25 g/l Ammonium acetate 0.25 g/l Yeast extract 0.25 g/l Na2-EDTA 0.003 g/l Resazurin 0.00225 g/l FeSO4 x 7 H2O 0.0011 g/l Vitamin B12 0.001 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 2 H2O 5e-05 g/l ZnCl2 4.2e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Na2MoO4 x 2 H2O 1.8e-05 g/l CuCl2 x 2 H2O 2e-06 g/l H2O Distilled water

culture temp

@refgrowthtypetemperature
5718positivegrowth30
67770positivegrowth30
5718positivegrowth28

Physiology and metabolism

oxygen tolerance

  • @ref: 5718
  • oxygen tolerance: anaerobe

spore formation

@refspore formationconfidence
125438no90.847
125439no98.7

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
5718microbial mat in marine salternRhone DeltaFranceFRAEurope
67770Microbial mat in marine saltern

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Microbial community#Microbial mat
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_107629.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15363;96_43192;97_55268;98_73146;99_107629&stattab=map
  • Last taxonomy: Halorhodospira neutriphila subclade
  • 16S sequence: AJ318525
  • Sequence Identity:
  • Total samples: 109
  • soil counts: 7
  • aquatic counts: 87
  • animal counts: 13
  • plant counts: 2

Interaction and safety

risk assessment

  • @ref: 5718
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

  • @ref: 66792
  • description: ASM1658405v1 assembly for Halorhodospira neutriphila DSM 15116
  • assembly level: contig
  • INSDC accession: GCA_016584055
  • BV-BRC accession: 168379.4
  • IMG accession: 2994947087
  • NCBI tax ID: 168379
  • score: 14.57

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Halorhodospira neutriphila gene for 16S ribosomal RNA, partial sequence, strain: JCM 17835AB6434731507nuccore168379
20218Halorhodospira neutrophila partial 16S rRNA gene, strain SG3301AJ3185251356nuccore168379

GC content

  • @ref: 67770
  • GC-content: 74.5
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingno98.75no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)yes52.182no
125438spore-formingspore-formingAbility to form endo- or exosporesno90.847no
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no68.382yes
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno86.263no
125438motile2+flagellatedAbility to perform flagellated movementyes73.998no
125439BacteriaNetspore_formationAbility to form endo- or exosporesno98.7
125439BacteriaNetmotilityAbility to perform movementyes78.6
125439BacteriaNetgram_stainReaction to gram-stainingnegative91.3
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthobligate anaerobe85

Literature

@ref: 5718

culture collection no.: DSM 15116, SG 3301, JCM 17835

straininfo link

  • @ref: 126262
  • straininfo: 100679

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12656167Isolation and characterization of spirilloid purple phototrophic bacteria forming red layers in microbial mats of Mediterranean salterns: description of Halorhodospira neutriphila sp. nov. and emendation of the genus Halorhodospira.Hirschler-Rea A, Matheron R, Riffaud C, Moune S, Eatock C, Herbert RA, Willison JC, Caumette PInt J Syst Evol Microbiol10.1099/ijs.0.02226-02003Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Ecosystem, Gammaproteobacteria/*classification/genetics/*isolation & purification/metabolism, Hydrogen-Ion Concentration, Light, Mediterranean Region, Microscopy, Electron, Molecular Sequence Data, Phylogeny, Pigments, Biological/analysis, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Seawater/microbiology, Sodium Chloride/isolation & purification, Sulfur/metabolismEnzymology
Enzymology22185719Prokaryotic diversity in Aran-Bidgol salt lake, the largest hypersaline playa in Iran.Makhdoumi-Kakhki A, Amoozegar MA, Kazemi B, Pasic L, Ventosa A.Microbes Environ10.1264/jsme2.me112672012isolation & purification, isolation & purification, Biodiversity, microbiologyPhylogeny
Genetics33375353Osmotic Adaptation and Compatible Solute Biosynthesis of Phototrophic Bacteria as Revealed from Genome Analyses.Imhoff JF, Rahn T, Kunzel S, Keller A, Neulinger SC.Microorganisms10.3390/microorganisms90100462020Osmotic Adaptation, Ectoine Biosynthesis, Genomes Of Photosynthetic Bacteria, Glycine Betaine Biosynthesis, Phylogeny Of Osmolyte BiosynthesisPhylogeny
Phylogeny35208750Genomic Comparison, Phylogeny and Taxonomic Reevaluation of the Ectothiorhodospiraceae and Description of Halorhodospiraceae fam. nov. and Halochlorospira gen. nov.Imhoff JF, Kyndt JA, Meyer TE.Microorganisms10.3390/microorganisms100202952022Purple Sulfur Bacteria, Ectothiorhodospiraceae, Halorhodospiraceae, Genomic Phylogeny, New Family And Genus

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5718Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15116)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15116
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann10.1101/2024.08.12.607695Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardyhttps://github.com/GenomeNet/deepGdeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1
126262A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer10.1093/database/baaf059StrainInfo—the central database for linked microbial strain identifiers