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BacDive ID 4655
Type strain
Strain Designation K12 AB1621
Culture col. no. DSM 7144 ATCC 25290 K12 AB1621
NCBI tax ID(s) 562
Links
version 10 (current version)

General

@ref: 3018

BacDive-ID: 4655

DSM-Number: 7144

keywords: mesophilic

description: Escherichia coli K12 AB1621 is a mesophilic prokaryote of the family Enterobacteriaceae.

NCBI tax id

  • NCBI tax id: 562
  • Matching level: species

strain history

  • @ref: 3018
  • history: <- ATCC <- E.A. Adelberg

doi: 10.13145/bacdive4655.20251217.10

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: kingdom/pseudomonadati
  • keyword: phylum/pseudomonadota
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Escherichia
  • species: Escherichia coli
  • full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Bacillus coli

@ref: 3018

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Escherichia

species: Escherichia coli

full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919

strain designation: K12 AB1621

type strain: no

Culture and growth conditions

culture medium

  • @ref: 3018
  • name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/381
  • composition: Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381; with strain-specific modifications) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Glucose 2.0 g/l Distilled water

culture temp

  • @ref: 3018
  • growth: positive
  • type: growth
  • temperature: 30

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836830849L-arabinose-fermentation
6836827613amygdalin-fermentation
6836828053melibiose-fermentation
6836817992sucrose-fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol+fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol-fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan+energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine+degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleyes
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin-
6836835581indole+

enzymes

@refvalueactivityec
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
3018+-+-----+--+--++----
3018+-+-----+--+--++-----

Interaction and safety

risk assessment

  • @ref: 3018
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Literature

@ref: 3018

culture collection no.: DSM 7144, ATCC 25290

straininfo link

  • @ref: 126262
  • straininfo: 38468

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism2667464Use of a novel air separation system in a fed-batch fermentative culture of Escherichia coli.Fass R, Clem TR, Shiloach J.Appl Environ Microbiol10.1128/aem.55.5.1305-1307.19891989growth & development, metabolism, Fermentation
Enzymology4590469Superoxide dismutase and oxygen toxicity in a eukaryote.Gregory EM, Goscin SA, Fridovich I.J Bacteriol10.1128/jb.117.2.456-460.19741974enzymology, Oxygen, metabolismMetabolism
Metabolism12874338Diminished adhesion of Anaplasma phagocytophilum-infected neutrophils to endothelial cells is associated with reduced expression of leukocyte surface selectin.Choi KS, Garyu J, Park J, Dumler JS.Infect Immun10.1128/iai.71.8.4586-4594.20032003immunology, microbiology, immunology, microbiology, pathogenicity, physiology, metabolism, metabolism, physiology
14576099Synthesis, degradation, and antimicrobial properties of targeted macromolecular prodrugs of norfloxacin.Roseeuw E, Coessens V, Balazuc AM, Lagranderie M, Chavarot P, Pessina A, Neri MG, Schacht E, Marchal G, Domurado D.Antimicrob Agents Chemother10.1128/aac.47.11.3435-3441.20032003chemical synthesis, pharmacology, chemical synthesis, pharmacology, chemical synthesis, pharmacology
Metabolism25261511Thiouracil-Forming Bacteria Identified and Characterized upon Porcine In Vitro Digestion of Brassicaceae Feed.Kiebooms JA, Wauters J, Vanden Bussche J, Houf K, De Vos P, Van Trappen S, Cleenwerck I, Vanhaecke L.Appl Environ Microbiol10.1128/aem.02370-142014microbiology, metabolism, metabolism, metabolism, Digestion, metabolism

Reference

@idauthorscataloguedoi/urltitle
3018Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7144)https://www.dsmz.de/collection/catalogue/details/culture/DSM-7144
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
68368Automatically annotated from API 20E
126262A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer10.1093/database/baaf059StrainInfo—the central database for linked microbial strain identifiers