Cite the latest
BacDive paper
×

Rate our new design

Thank you!
BacDive D3 OLD DESIGN
Content
Advanced search Isolation sources API ® test finder TAXplorer Statistics Special collections Download selection Web services SPARQL
About us
About Team Contact Tutorials News Mailinglist
BacDive ID 6866
Type strain
Strain Designation S3, S3
Culture col. no. DSM 4712 ATCC 43754 CIP 102920 S3 S3
NCBI tax ID(s) 2757
Links
For citation purpose refer to the digital object identifier (doi) of the current version.

Archive

version 9.2 (current version):
version 9.2 (current version)

General

@ref: 1727

BacDive-ID: 6866

DSM-Number: 4712

keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, rod-shaped

description: Brochothrix campestris S3 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 2757
  • Matching level: species

strain history

@refhistory
1727<- CIP <- Talon et al., S3
412011987, R. Talon: strain S3
117114CIP <- 1987, R. Talon, INRA, Ceyrat, France: strain S3

doi: 10.13145/bacdive6866.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Listeriaceae
  • genus: Brochothrix
  • species: Brochothrix campestris
  • full scientific name: Brochothrix campestris Talon et al. 1988

@ref: 1727

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Listeriaceae

genus: Brochothrix

species: Brochothrix campestris

full scientific name: Brochothrix campestris Talon et al. 1988

strain designation: S3, S3

type strain: yes

Morphology

cell morphology

  • @ref: 117114
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1727TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
41201MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
117114CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperature
1727positivegrowth26
41201positivegrowth22
117114positivegrowth15-37

Physiology and metabolism

oxygen tolerance

  • @ref: 117114
  • oxygen tolerance: facultative anaerobe

halophily

@refsaltgrowthtested relationconcentration
117114NaClpositivegrowth0-4 %
117114NaClnogrowth6 %
117114NaClnogrowth8 %
117114NaClnogrowth10 %

murein

  • @ref: 1727
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
117114606565hippurate+hydrolysis
11711417632nitrate-reduction
11711416301nitrite-reduction
11711417632nitrate+respiration
6837118305arbutin+builds acid from
6837127613amygdalin+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose+builds acid from
6837128066gentiobiose+builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837159640N-acetylglucosamine+builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose+builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol+builds acid from

metabolite production

  • @ref: 117114
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11711415688acetoin+
11711417234glucose+

enzymes

@refvalueactivityec
117114oxidase-
117114alcohol dehydrogenase-1.1.1.1
117114catalase+1.11.1.6
117114lysine decarboxylase-4.1.1.18
117114ornithine decarboxylase-4.1.1.17
117114urease-3.5.1.5

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
117114+---+-----+++-+--+---+++++++---+------++------+/---

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
1727soil
117114Environment, SoilFranceFRAEurope

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_264.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_32;96_185;97_201;98_224;99_264&stattab=map
  • Last taxonomy: Brochothrix
  • 16S sequence: AY543038
  • Sequence Identity:
  • Total samples: 40556
  • soil counts: 3704
  • aquatic counts: 5597
  • animal counts: 29797
  • plant counts: 1458

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
17271Risk group (German classification)
1171141Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218B.campestris gene for 16S ribosomal RNAX561561480nuccore2757
20218Brochothrix campestris isolate DSMZ 4712 16S ribosomal RNA gene, partial sequenceAY5430381420nuccore2757
20218Brochothrix campestris gene for 16S rRNA, partial sequence, strain: NBRC 15547AB6808971473nuccore2757

GC content

  • @ref: 1727
  • GC-content: 38.0

External links

@ref: 1727

culture collection no.: DSM 4712, ATCC 43754, CIP 102920

straininfo link

  • @ref: 76331
  • straininfo: 92132

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Biotechnology8357264Brochocin-C, a new bacteriocin produced by Brochothrix campestris.Siragusa GR, Cutter CNAppl Environ Microbiol10.1128/aem.59.7.2326-2328.19931993*Antibiosis, Bacteriocins/*biosynthesis, *Food Microbiology, Gram-Positive Rods/*metabolism, Microbial Sensitivity TestsMetabolism
Pathogenicity9835559Genetic characterization and heterologous expression of brochocin-C, an antibotulinal, two-peptide bacteriocin produced by Brochothrix campestris ATCC 43754.McCormick JK, Poon A, Sailer M, Gao Y, Roy KL, McMullen LM, Vederas JC, Stiles ME, Van Belkum MJAppl Environ Microbiol10.1128/AEM.64.12.4757-4766.19981998Amino Acid Sequence, Animals, Bacteriocins/chemistry/*genetics/pharmacology, Base Sequence, Genes, Bacterial, Genetic Complementation Test, Gram-Negative Bacteria/drug effects, Gram-Positive Bacteria/drug effects/*genetics, Meat/microbiology, Microbial Sensitivity Tests, Molecular Sequence Data, Restriction MappingGenetics
Pathogenicity10508055The outer membrane of gram-negative bacteria inhibits antibacterial activity of brochocin-C.Gao Y, van Belkum MJ, Stiles MEAppl Environ Microbiol10.1128/AEM.65.10.4329-4333.19991999Adenosine Triphosphate/metabolism, Bacteriocins/*pharmacology, Cell Membrane/drug effects/physiology, Edetic Acid/pharmacology, Gram-Negative Bacteria/*drug effects, Hydrolysis, Nisin/pharmacologyMetabolism
Metabolism12620816Purification and characterization of brochocin A and brochocin B(10-43), a functional fragment generated by heterologous expression in Carnobacterium piscicola.Garneau S, Ference CA, van Belkum MJ, Stiles ME, Vederas JCAppl Environ Microbiol10.1128/AEM.69.3.1352-1358.20032003Amino Acid Sequence, *Bacteriocins/genetics/isolation & purification/metabolism/pharmacology, Base Sequence, Circular Dichroism, Culture Media, Escherichia coli/genetics/metabolism, Food Preservatives, Lactobacillaceae/drug effects/genetics/*metabolism, Mass Spectrometry/methods, Microbial Sensitivity Tests, Molecular Sequence Data, Sequence Analysis, DNAEnzymology
Biotechnology16943083Control of meatborne Listeria monocytogenes and Brochothrix thermosphacta by a bacteriocinogenic Brochothrix campestris ATCC 43754.Greer GG, Dilts BDFood Microbiol10.1016/j.fm.2006.02.0092006Adipose Tissue/microbiology, Animals, Anti-Bacterial Agents, *Antibiosis, Bacteriocins/*biosynthesis, Coculture Techniques, Colony Count, Microbial, Food Microbiology, Gram-Positive Rods/*growth & development/*physiology, Humans, Listeria monocytogenes/*growth & development, Meat/*microbiology/standards, Odorants/analysis, Swine, Taste, Temperature, Time FactorsPathogenicity
Enzymology22738965Purification and characterization of multiple bacteriocins and an inducing peptide produced by Enterococcus faecium NKR-5-3 from Thai fermented fish.Ishibashi N, Himeno K, Fujita K, Masuda Y, Perez RH, Zendo T, Wilaipun P, Leelawatcharamas V, Nakayama J, Sonomoto KBiosci Biotechnol Biochem10.1271/bbb.1109722012Amino Acid Sequence, Animals, Bacteriocins/biosynthesis/genetics/*isolation & purification, Base Sequence, Chromatography, Reverse-Phase, Enterococcus faecium/genetics/*metabolism, Fermentation, Fishes/*microbiology, Lactobacillus/drug effects/metabolism, Microbial Sensitivity Tests, Microbial Viability/drug effects, Molecular Sequence Data, Peptides/genetics/*isolation & purification/metabolism, Recombinant Proteins/biosynthesis/genetics/isolation & purification, Sequence Analysis, DNAPhylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1727Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4712)https://www.dsmz.de/collection/catalogue/details/culture/DSM-4712
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41201Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14660
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68371Automatically annotated from API 50CH acid
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76331Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92132.1StrainInfo: A central database for resolving microbial strain identifiers
117114Curators of the CIPCollection of Institut Pasteur (CIP 102920)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102920

BacDive

About Team News Mailinglist

Help

How to cite Tutorials Contact Help

Social Media

BlueskyBluesky linkedin de.NBI Survey
DSMZ Global core biodata resource deNBI Elixis core data resource DZIF NFDI4 Biodiversity NFDI4 Microbiota
© DSMZ 2025 Imprint Privacy Statement Copyright & License License