Cite this strain

Marinactinospora thermotolerans (DSM 45154, CCTCC AA 208041, JCM 16903)

Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 11263

Type strain

Culture col. no. DSM 45154 CCTCC AA 208041 JCM 16903 SCSIO 00652 NBRC 110046

NCBI tax ID(s) 1122192 531310

Special Collections DSMZ JCM Literature strains

Links

Name and taxonomic classification

Marinactinospora thermotolerans DSM 45154 is an aerobe, spore-forming, mesophilic bacterium that was isolated from deep-sea sediment at about 3865 m depth.

spore-forming Gram-positive motile aerobe mesophilic genome sequence 16S sequence Bacteria

SI-ID 405821

DSM 45154

Last LPSN update 2024-12-09 10:44:20

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Streptosporangiales

Family Nocardiopsidaceae

Genus Marinactinospora

Species Marinactinospora thermotolerans

Full scientific name Marinactinospora thermotolerans Tian et al. 2009

Morphology

Cell morphology

@ref	Gram stain	Motility
29062	positive
125439	positive
125439

Multimedia

@ref:	15448
manual_annotation:	1
multimedia content:	DSM_45154.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_45154.jpg
caption:	Medium 535 28°C
intellectual property rights:	© Leibniz-Institut DSMZ

@ref:	66793
manual_annotation:	1
multimedia content:	EM_DSM_45154_1.jpg
multimedia.multimedia content:	EM_DSM_45154_1.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde

@ref:	66793
manual_annotation:	1
multimedia content:	EM_DSM_45154_2.jpg
multimedia.multimedia content:	EM_DSM_45154_2.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde

@ref:	66793
manual_annotation:	1
multimedia content:	EM_DSM_45154_3.jpg
multimedia.multimedia content:	EM_DSM_45154_3.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde

@ref:	66793
manual_annotation:	1
multimedia content:	EM_DSM_45154_4.jpg
multimedia.multimedia content:	EM_DSM_45154_4.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde

@ref:	66793
manual_annotation:	1
multimedia content:	EM_DSM_45154_5.jpg
multimedia.multimedia content:	EM_DSM_45154_5.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde

Pigmentation

29062

Productionno

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
15448	GPHF-MEDIUM (DSMZ Medium 553)		Medium recipe at MediaDive	Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
15448	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)		Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
15448	positive	growth	28	mesophilic
29062	positive	growth	10-55
29062	positive	optimum	28	mesophilic
67770	positive	growth	28	mesophilic

Culture pH

@ref	Ability	Type	PH
29062	positive	optimum	7.5
29062	positive	growth	06-09

Physiology and metabolism

Halophily

@ref	Salt	Growth	Tested relation	Concentration
29062	NaCl	positive	growth	0-5 %
29062	NaCl	positive	optimum	0.5 %

Observation

@ref	Observation
29062	aggregates in chains
67770	quinones: MK-10(H₈), MK-11(H₈), MK-11(H₁₀)

Spore formation

@ref	Spore formation
29062
125439

Enzymes

@ref	Value	Activity	Ec
29062	catalase	+	1.11.1.6
29062	cytochrome oxidase	+	1.9.3.1
29062	urease	+	3.5.1.5

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
29062	17057 ChEBI	cellobiose	+	carbon source
29062	28757 ChEBI	fructose	+	carbon source
29062	28260 ChEBI	galactose	+	carbon source
29062	17234 ChEBI	glucose	+	carbon source
29062	17306 ChEBI	maltose	+	carbon source
29062	29864 ChEBI	mannitol	+	carbon source
29062	37684 ChEBI	mannose	+	carbon source
29062	17632 ChEBI	nitrate	+	reduction
29062	26546 ChEBI	rhamnose	+	carbon source
29062	33942 ChEBI	ribose	+	carbon source
29062	30911 ChEBI	sorbitol	+	carbon source
29062	17992 ChEBI	sucrose	+	carbon source
29062	53424 ChEBI	tween 20	+	carbon source
29062	53423 ChEBI	tween 40	+	carbon source
29062	53425 ChEBI	tween 60	+	carbon source
29062	53426 ChEBI	tween 80	+	carbon source
29062	16199 ChEBI	urea	+	carbon source
29062	18222 ChEBI	xylose	+	carbon source

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	cellulose degradation	100	5 of 5
66794	ppGpp biosynthesis	100	4 of 4
66794	adipate degradation	100	2 of 2
66794	molybdenum cofactor biosynthesis	100	9 of 9
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	enterobactin biosynthesis	100	3 of 3
66794	starch degradation	100	10 of 10
66794	palmitate biosynthesis	100	22 of 22
66794	cardiolipin biosynthesis	100	7 of 7
66794	acetate fermentation	100	4 of 4
66794	aspartate and asparagine metabolism	100	9 of 9
66794	4-hydroxymandelate degradation	100	9 of 9
66794	threonine metabolism	100	10 of 10
66794	glycogen metabolism	100	5 of 5
66794	chorismate metabolism	100	9 of 9
66794	phenylacetate degradation (aerobic)	100	5 of 5
66794	resorcinol degradation	100	2 of 2
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	suberin monomers biosynthesis	100	2 of 2
66794	folate polyglutamylation	100	1 of 1
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	coenzyme A metabolism	100	4 of 4
66794	formaldehyde oxidation	100	3 of 3
66794	glycine betaine biosynthesis	100	5 of 5
66794	vitamin K metabolism	100	5 of 5
66794	methylglyoxal degradation	100	5 of 5
66794	glycolate and glyoxylate degradation	100	6 of 6
66794	ethanol fermentation	100	2 of 2
66794	urea cycle	92.31	12 of 13
66794	phenylalanine metabolism	92.31	12 of 13
66794	leucine metabolism	92.31	12 of 13
66794	pentose phosphate pathway	90.91	10 of 11
66794	Entner Doudoroff pathway	90	9 of 10
66794	glutamate and glutamine metabolism	89.29	25 of 28
66794	valine metabolism	88.89	8 of 9
66794	serine metabolism	88.89	8 of 9
66794	CO2 fixation in Crenarchaeota	88.89	8 of 9
66794	C4 and CAM-carbon fixation	87.5	7 of 8
66794	gluconeogenesis	87.5	7 of 8
66794	peptidoglycan biosynthesis	86.67	13 of 15
66794	citric acid cycle	85.71	12 of 14
66794	heme metabolism	85.71	12 of 14
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	photosynthesis	85.71	12 of 14
66794	propanol degradation	85.71	6 of 7
66794	purine metabolism	84.04	79 of 94
66794	NAD metabolism	83.33	15 of 18
66794	selenocysteine biosynthesis	83.33	5 of 6
66794	metabolism of amino sugars and derivatives	80	4 of 5
66794	factor 420 biosynthesis	80	4 of 5
66794	flavin biosynthesis	80	12 of 15
66794	propionate fermentation	80	8 of 10
66794	vitamin B12 metabolism	79.41	27 of 34
66794	glutathione metabolism	78.57	11 of 14
66794	d-mannose degradation	77.78	7 of 9
66794	allantoin degradation	77.78	7 of 9
66794	vitamin B1 metabolism	76.92	10 of 13
66794	glycolysis	76.47	13 of 17
66794	histidine metabolism	75.86	22 of 29
66794	alanine metabolism	75.86	22 of 29
66794	pyrimidine metabolism	75.56	34 of 45
66794	cyclohexanol degradation	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	sulfopterin metabolism	75	3 of 4
66794	butanoate fermentation	75	3 of 4
66794	isoleucine metabolism	75	6 of 8
66794	arginine metabolism	75	18 of 24
66794	glycogen biosynthesis	75	3 of 4
66794	lipid metabolism	74.19	23 of 31
66794	non-pathway related	73.68	28 of 38
66794	methionine metabolism	73.08	19 of 26
66794	metabolism of disaccharids	72.73	8 of 11
66794	proline metabolism	72.73	8 of 11
66794	degradation of sugar acids	72	18 of 25
66794	tetrahydrofolate metabolism	71.43	10 of 14
66794	ubiquinone biosynthesis	71.43	5 of 7
66794	tryptophan metabolism	71.05	27 of 38
66794	oxidative phosphorylation	70.33	64 of 91
66794	myo-inositol biosynthesis	70	7 of 10
66794	lysine metabolism	69.05	29 of 42
66794	degradation of sugar alcohols	68.75	11 of 16
66794	degradation of pentoses	67.86	19 of 28
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	cyanate degradation	66.67	2 of 3
66794	methane metabolism	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	isoprenoid biosynthesis	65.38	17 of 26
66794	tyrosine metabolism	64.29	9 of 14
66794	vitamin B6 metabolism	63.64	7 of 11
66794	d-xylose degradation	63.64	7 of 11
66794	ketogluconate metabolism	62.5	5 of 8
66794	cysteine metabolism	61.11	11 of 18
66794	gallate degradation	60	3 of 5
66794	lipoate biosynthesis	60	3 of 5
66794	3-chlorocatechol degradation	60	3 of 5
66794	glycine metabolism	60	6 of 10
66794	hydrogen production	60	3 of 5
66794	aclacinomycin biosynthesis	57.14	4 of 7
66794	carotenoid biosynthesis	54.55	12 of 22
66794	grixazone biosynthesis	50	1 of 2
66794	lactate fermentation	50	2 of 4
66794	toluene degradation	50	2 of 4
66794	ribulose monophosphate pathway	50	1 of 2
66794	mannosylglycerate biosynthesis	50	1 of 2
66794	pantothenate biosynthesis	50	3 of 6
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	CMP-KDO biosynthesis	50	2 of 4
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	phenylmercury acetate degradation	50	1 of 2
66794	dTDPLrhamnose biosynthesis	50	4 of 8
66794	biotin biosynthesis	50	2 of 4
66794	vitamin E metabolism	50	2 of 4
66794	degradation of hexoses	50	9 of 18
66794	quinate degradation	50	1 of 2
66794	polyamine pathway	47.83	11 of 23
66794	phenylpropanoid biosynthesis	46.15	6 of 13
66794	phosphatidylethanolamine bioynthesis	46.15	6 of 13
66794	sulfate reduction	46.15	6 of 13
66794	ascorbate metabolism	45.45	10 of 22
66794	phenol degradation	45	9 of 20
66794	daunorubicin biosynthesis	44.44	4 of 9
66794	nitrate assimilation	44.44	4 of 9
66794	lipid A biosynthesis	44.44	4 of 9
66794	androgen and estrogen metabolism	43.75	7 of 16
66794	bile acid biosynthesis, neutral pathway	41.18	7 of 17
66794	arachidonate biosynthesis	40	2 of 5
66794	elloramycin biosynthesis	40	2 of 5
66794	ethylmalonyl-CoA pathway	40	2 of 5
66794	3-phenylpropionate degradation	40	6 of 15
66794	carnitine metabolism	37.5	3 of 8
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	1,4-dihydroxy-6-naphthoate biosynthesis	33.33	2 of 6
66794	sphingosine metabolism	33.33	2 of 6
66794	sulfoquinovose degradation	33.33	1 of 3
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	coenzyme M biosynthesis	30	3 of 10
66794	benzoyl-CoA degradation	28.57	2 of 7
66794	arachidonic acid metabolism	27.78	5 of 18
66794	chlorophyll metabolism	22.22	4 of 18

Oxygen tolerance

@ref	Oxygen tolerance
29062	aerobe
125439	obligate aerobe

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude
15448	deep-sea sediment at about 3865 m depth	northern South China Sea (17° 58.742' N 116° 00.228' E)	China	CHN	Asia	17.9792	116.004 17.9792/116.004
67770	Deep-sea sediment (black soft mud at a depth of 3,865 m)		China	CHN	Asia

Taxonmaps

69479

Global distribution of 16S sequence EU698029 (>99% sequence identity) for Marinactinospora from Microbeatlas

Aquatic Samples	18
Soil Samples	219
Animal Samples	52
Plant Samples	72
Total Samples	361

Safety information

Risk assessment

@ref	Biosafety level	Biosafety level comment
15448	1	Risk group (German classification) According to TRBA 466

Sequence information

GC content

@ref	GC-content (mol%)	Method
15448	72	high performance liquid chromatography (HPLC)
29062	72

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
15448	Marinactinospora thermotolerans strain SCSIO 00652 16S ribosomal RNA gene, partial sequence	EU698029	1443		531310

Genome sequences

@ref	Description	Accession	Assembly level	Database	NCBI tax ID
66792	Marinactinospora thermotolerans DSM 45154	1122192.6	wgs	PATRIC	1122192
66792	Marinactinospora thermotolerans DSM 45154	2524614583	draft	JGI IMG/M	1122192
67770	Marinactinospora thermotolerans DSM 45154	GCA_900167435	scaffold		1122192

Genome-based predictions

Predictions

Predictions from Diaspora project based on:
Marinactinospora thermotolerans DSM 45154
NCBI: GCA_900167435

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	93.90	no
125439	motility	BacteriaNetⓘ	no	92.40	no
125439	gram_stain	BacteriaNetⓘ	positive	99.50	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	97.90	no

Predictions from Diaspora project based on:
Marinactinospora thermotolerans DSM 45154
PATRIC: 1122192.6

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	84.17	yes
125438	anaerobic	anaerobicⓘ	no	95.93	yes
125438	spore-forming	spore-formingⓘ	yes	80.77	no
125438	aerobic	aerobicⓘ	yes	89.60	yes
125438	thermophilic	thermophileⓘ	no	92.88	no
125438	flagellated	motile2+ⓘ	no	88.00	no

External links

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Characterization of MtdV as a chorismate lyase essential to A201A biosynthesis and precursor-directed biosynthesis of new analogs.	Zhu Q, Song Y, Huang H, Li Q, Ju J	Org Biomol Chem	10.1039/c8ob02852d	2019
Phylogeny	Mathermycin, a Lantibiotic from the Marine Actinomycete Marinactinospora thermotolerans SCSIO 00652.	Chen E, Chen Q, Chen S, Xu B, Ju J, Wang H	Appl Environ Microbiol	10.1128/AEM.00926-17	2017
Enzymology	Functional Characterization of a Novel Marine Microbial GDSL Lipase and Its Utilization in the Resolution of (+/-)-1-Phenylethanol.	Deng D, Zhang Y, Sun A, Liang J, Hu Y	Appl Biochem Biotechnol	10.1007/s12010-016-1980-4	2016
Enzymology	Marthiapeptide A, an anti-infective and cytotoxic polythiazole cyclopeptide from a 60 L scale fermentation of the deep sea-derived Marinactinospora thermotolerans SCSIO 00652.	Zhou X, Huang H, Chen Y, Tan J, Song Y, Zou J, Tian X, Hua Y, Ju J	J Nat Prod	10.1021/np300554f	2012
Metabolism	Characterization of a single gene cluster responsible for methylpendolmycin and pendolmycin biosynthesis in the deep sea bacterium Marinactinospora thermotolerans.	Ma J, Zuo D, Song Y, Wang B, Huang H, Yao Y, Li W, Zhang S, Zhang C, Ju J	Chembiochem	10.1002/cbic.201100700	2012
Genetics	Discovery and engineered overproduction of antimicrobial nucleoside antibiotic A201A from the deep-sea marine actinomycete Marinactinospora thermotolerans SCSIO 00652.	Zhu Q, Li J, Ma J, Luo M, Wang B, Huang H, Tian X, Li W, Zhang S, Zhang C, Ju J	Antimicrob Agents Chemother	10.1128/AAC.05278-11	2011
Enzymology	Antimalarial beta-carboline and indolactam alkaloids from Marinactinospora thermotolerans, a deep sea isolate.	Huang H, Yao Y, He Z, Yang T, Ma J, Tian X, Li Y, Huang C, Chen X, Li W, Zhang S, Zhang C, Ju J	J Nat Prod	10.1021/np200399t	2011
Phylogeny	Marinitenerispora sediminis gen. nov., sp. nov., a member of the family Nocardiopsaceae isolated from marine sediment.	Ng ZY, Fang BZ, Li WJ, Tan GYA	Int J Syst Evol Microbiol	10.1099/ijsem.0.003587	2019
Phylogeny	Marinactinospora rubrisoli sp. nov., isolated from red soil.	Huang B, Cheng K, Zhang L, Huang Y, Liu C	Arch Microbiol	10.1007/s00203-018-1583-2	2018
Phylogeny	Marinactinospora endophytica sp. nov., isolated from a medicinal plant.	Liu MJ, Khieu TN, Gao R, Hozzein WN, Wang HF, Yang W, Nimaichand S, Xiong Z, Duan YQ, Li WJ	Antonie Van Leeuwenhoek	10.1007/s10482-015-0451-1	2015
Phylogeny	Spinactinospora alkalitolerans gen. nov., sp. nov., an actinomycete isolated from marine sediment.	Chang X, Liu W, Zhang XH	Int J Syst Evol Microbiol	10.1099/ijs.0.027383-0	2011
Phylogeny	Marinactinospora thermotolerans gen. nov., sp. nov., a marine actinomycete isolated from a sediment in the northern South China Sea.	Tian XP, Tang SK, Dong JD, Zhang YQ, Xu LH, Zhang S, Li WJ	Int J Syst Evol Microbiol	10.1099/ijs.0.005231-0	2009

Straininfo Link

@ref	Straininfo	Culture collections
80496	405821	DSM 45154

Culture collection no.

Culture collection no.
DSM 45154
CCTCC AA 208041
JCM 16903
SCSIO 00652
NBRC 110046

References

#15448	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45154
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#29062	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25492 (see below)
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66793	Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#80496	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405821.1 )
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

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