Cite this strain

Bombilactobacillus mellifer (DSM 26254, CCUG 63291, Bin4N)

Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 130370

Type strain

Strain Designation Bin4N

Culture col. no. DSM 26254 CCUG 63291 Bin4N JCM 30768

NCBI tax ID(s) 1218492

Special Collections DSMZ CCUG JCM

Links

Name and taxonomic classification

Bombilactobacillus mellifer Bin4N is an anaerobe, mesophilic bacterium that was isolated from honey stomach of honey bee .

anaerobe mesophilic 16S sequence Bacteria

SI-ID 397283

DSM 26254, CCUG 63291

Last LPSN update 2024-12-09 10:48:56

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Bombilactobacillus

Species Bombilactobacillus mellifer

Full scientific name Bombilactobacillus mellifer (Olofsson et al. 2014) Zheng et al. 2020

Synonyms (1)

Lactobacillus mellifer

BacDive ID	Other strains from **Bombilactobacillus mellifer** (1)	Type strain
130371	B. mellifer Bin4, DSM 26316, CCUG 65819

Morphology

Colony morphology

62967

Incubation period3 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
21906	MRS MEDIUM (DSMZ Medium 11)		Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11; with strain-specific modifications) Composition: Fructose 20.0 g/l Glucose 20.0 g/l Meat extract 10.0 g/l Casein peptone 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
21906	positive	growth	35	mesophilic
62967	positive	growth	37	mesophilic
67770	positive	growth	37	mesophilic

Physiology and metabolism

Murein

@ref	Murein short key	Type
21906	A11.31	A4alpha L-Lys-D-Asp

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	gluconeogenesis	100	8 of 8
66794	acetate fermentation	100	4 of 4
66794	palmitate biosynthesis	100	22 of 22
66794	ppGpp biosynthesis	100	4 of 4
66794	pentose phosphate pathway	100	11 of 11
66794	ribulose monophosphate pathway	100	2 of 2
66794	formaldehyde oxidation	100	3 of 3
66794	coenzyme A metabolism	100	4 of 4
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	suberin monomers biosynthesis	100	2 of 2
66794	folate polyglutamylation	100	1 of 1
66794	acetoin degradation	100	3 of 3
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	degradation of sugar alcohols	93.75	15 of 16
66794	C4 and CAM-carbon fixation	87.5	7 of 8
66794	peptidoglycan biosynthesis	86.67	13 of 15
66794	vitamin B12 metabolism	82.35	28 of 34
66794	Entner Doudoroff pathway	80	8 of 10
66794	photosynthesis	78.57	11 of 14
66794	aspartate and asparagine metabolism	77.78	7 of 9
66794	ketogluconate metabolism	75	6 of 8
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	vitamin B1 metabolism	69.23	9 of 13
66794	purine metabolism	69.15	65 of 94
66794	NAD metabolism	66.67	12 of 18
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	valine metabolism	66.67	6 of 9
66794	serine metabolism	66.67	6 of 9
66794	CO2 fixation in Crenarchaeota	66.67	6 of 9
66794	selenocysteine biosynthesis	66.67	4 of 6
66794	glycolysis	64.71	11 of 17
66794	phenylalanine metabolism	61.54	8 of 13
66794	cellulose degradation	60	3 of 5
66794	myo-inositol biosynthesis	60	6 of 10
66794	glycogen metabolism	60	3 of 5
66794	pyrimidine metabolism	57.78	26 of 45
66794	methionine metabolism	53.85	14 of 26
66794	oxidative phosphorylation	53.85	49 of 91
66794	degradation of pentoses	53.57	15 of 28
66794	threonine metabolism	50	5 of 10
66794	dTDPLrhamnose biosynthesis	50	4 of 8
66794	glycolate and glyoxylate degradation	50	3 of 6
66794	isoleucine metabolism	50	4 of 8
66794	sulfopterin metabolism	50	2 of 4
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	butanoate fermentation	50	2 of 4
66794	glutamate and glutamine metabolism	50	14 of 28
66794	ethanol fermentation	50	1 of 2
66794	mannosylglycerate biosynthesis	50	1 of 2
66794	urea cycle	46.15	6 of 13
66794	proline metabolism	45.45	5 of 11
66794	lysine metabolism	45.24	19 of 42
66794	alanine metabolism	44.83	13 of 29
66794	d-mannose degradation	44.44	4 of 9
66794	degradation of hexoses	44.44	8 of 18
66794	degradation of sugar acids	44	11 of 25
66794	heme metabolism	42.86	6 of 14
66794	propanol degradation	42.86	3 of 7
66794	mevalonate metabolism	42.86	3 of 7
66794	isoprenoid biosynthesis	42.31	11 of 26
66794	non-pathway related	42.11	16 of 38
66794	arginine metabolism	41.67	10 of 24
66794	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
66794	methylglyoxal degradation	40	2 of 5
66794	glycine metabolism	40	4 of 10
66794	ethylmalonyl-CoA pathway	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	lipoate biosynthesis	40	2 of 5
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	37.5	3 of 8
66794	vitamin B6 metabolism	36.36	4 of 11
66794	metabolism of disaccharids	36.36	4 of 11
66794	tetrahydrofolate metabolism	35.71	5 of 14
66794	citric acid cycle	35.71	5 of 14
66794	lipid metabolism	35.48	11 of 31
66794	cyanate degradation	33.33	1 of 3
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	cysteine metabolism	33.33	6 of 18
66794	IAA biosynthesis	33.33	1 of 3
66794	octane oxidation	33.33	1 of 3
66794	sphingosine metabolism	33.33	2 of 6
66794	enterobactin biosynthesis	33.33	1 of 3
66794	leucine metabolism	30.77	4 of 13
66794	propionate fermentation	30	3 of 10
66794	starch degradation	30	3 of 10
66794	tryptophan metabolism	28.95	11 of 38
66794	reductive acetyl coenzyme A pathway	28.57	2 of 7
66794	d-xylose degradation	27.27	3 of 11
66794	flavin biosynthesis	26.67	4 of 15
66794	lactate fermentation	25	1 of 4
66794	biotin biosynthesis	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	toluene degradation	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	CMP-KDO biosynthesis	25	1 of 4
66794	glycogen biosynthesis	25	1 of 4
66794	histidine metabolism	24.14	7 of 29
66794	sulfate reduction	23.08	3 of 13
66794	ascorbate metabolism	22.73	5 of 22
66794	nitrate assimilation	22.22	2 of 9
66794	lipid A biosynthesis	22.22	2 of 9
66794	glutathione metabolism	21.43	3 of 14
66794	tyrosine metabolism	21.43	3 of 14

Oxygen tolerance

@ref	Oxygen tolerance
21906	anaerobe
62967	anaerobe

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
21906	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	#Stomach

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent
21906	honey stomach of honey bee (Apis mellifera mellifera)	Apis mellifera mellifera	Lund	Sweden	SWE	Europe
62967	Honey bee,honey stomach		Helsingborg	Sweden	SWE	Europe
67770	Honey stomach of the honeybee Apis mellifera mellifera	Apis mellifera mellifera

Taxonmaps

69479

Global distribution of 16S sequence JX099543 (>99% sequence identity) for Bombilactobacillus mellifer subclade from Microbeatlas

Aquatic Samples	127
Soil Samples	38
Animal Samples	3619
Plant Samples	155
Total Samples	3939

Safety information

Risk assessment

@ref	Biosafety level	Biosafety level comment
21906	1	Risk group (German classification)

Sequence information

GC content

@ref	GC-content (mol%)	Method
21906	39.4	sequence analysis
67770	39.5	genome sequence analysis
67770	39.4	genome sequence analysis

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
21906	Lactobacillus mellifer strain Bin4N 16S ribosomal RNA gene, partial sequence	JX099543	1437		1218492

Genome-based predictions

External links

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Lactobacillus apinorum sp. nov., Lactobacillus mellifer sp. nov., Lactobacillus mellis sp. nov., Lactobacillus melliventris sp. nov., Lactobacillus kimbladii sp. nov., Lactobacillus helsingborgensis sp. nov. and Lactobacillus kullabergensis sp. nov., isolated from the honey stomach of the honeybee Apis mellifera.	Olofsson TC, Alsterfjord M, Nilson B, Butler E, Vasquez A	Int J Syst Evol Microbiol	10.1099/ijs.0.059600-0	2014
Phylogeny	Bombilactobacillus apium sp. nov., isolated from the gut of honeybee (Apis cerana).	Kang JP, Huo Y, Hoang VA, Yang DU, Yang DC, Kang SC	Arch Microbiol	10.1007/s00203-021-02249-y	2021

Straininfo Link

@ref	Straininfo	Culture collections
88902	397283	DSM 26254, CCUG 63291

Culture collection no.

Culture collection no.
DSM 26254
CCUG 63291
Bin4N
JCM 30768

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#21906	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 26254
#62967	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 63291
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#88902	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID397283.1 )

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