Cite this strain

Pseudoalteromonas distincta (DSM 12749, ATCC 700518, CIP 105340)

Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 131079

Type strain

Culture col. no. DSM 12749 ATCC 700518 CIP 105340 KMM 638 VKM B-2136 D

NCBI tax ID(s) 77608

Special Collections DSMZ CIP

Links

Name and taxonomic classification

Pseudoalteromonas distincta DSM 12749 is a psychrophilic, Gram-negative, motile bacterium that was isolated from Marine sponge from a depth of 350 m near the Komandorskiye Islands.

Gram-negative motile rod-shaped psychrophilic genome sequence 16S sequence Bacteria

SI-ID 46242

ATCC 700518, DSM 12749, CIP 105340

Last LPSN update 2024-12-09 10:49:00

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Alteromonadales

Family Pseudoalteromonadaceae

Genus Pseudoalteromonas

Species Pseudoalteromonas distincta

Full scientific name Pseudoalteromonas distincta (Romanenko et al. 1995) Ivanova et al. 2000

Synonyms (4)

Pseudoalteromonas elyakovii
Alteromonas elyakovii
Alteromonas distincta
Pseudoalteromonas paragorgicola

Morphology

Cell morphology

@ref	Gram stain	Confidence
125438	negative	100
125439	negative	99.4
118949	negative

Pigmentation

@ref	Production	Name
118949		Pyocyanin

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
22371	BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)	Medium recipe at MediaDive	Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
42037	Marine agar (MA)		Distilled water make up to (1000.000 ml);Marine agar (55.100 g)
118949	CIP Medium 13	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
22371	positive	growth	20	psychrophilic
42037	positive	growth	25	mesophilic
118949	positive	growth	5-30
118949	negative	growth	37
118949	negative	growth	41

Physiology and metabolism

Halophily

@ref	Salt	Growth	Tested relation	Concentration
118949	NaCl	positive	growth	4-6 %
118949	NaCl		growth	0 %
118949	NaCl		growth	2 %
118949	NaCl		growth	8 %
118949	NaCl		growth	10 %

Spore formation

@ref	Spore formation	Confidence
125439		99.3

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
118949	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
118949	amylase	+
118949	beta-galactosidase	-	3.2.1.23
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
118949	caseinase	+	3.4.21.50
118949	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	+		from API zym
118949	gelatinase	+/-
118949	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
118949	lipase	+
68382	lipase (C 14)	-		from API zym
118949	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
118949	ornithine decarboxylase	-	4.1.1.17
118949	oxidase	+
118949	phenylalanine ammonia-lyase	-	4.3.1.24
118949	protease	+
68382	trypsin	-	3.4.21.4	from API zym
118949	tryptophan deaminase	-
118949	tween esterase	+
118949	urease	-	3.5.1.5
68382	valine arylamidase	+		from API zym

Metabolite production

@ref	Chebi-ID	Metabolite	Production
118949	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
118949	15688 ChEBI	acetoin	-
118949	17234 ChEBI	glucose

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
118949	17632 ChEBI	nitrate	+	reduction
118949	16301 ChEBI	nitrite	-	reduction

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	98.5

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
118949	-	+	-	+	-	+	+	-	-	+	+	+	-	-	-	-	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
118949	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Marine
#Host	#Invertebrates (Other)	#Porifera (Sponges)

Isolation

@ref	Sample type	Country	Country ISO 3 Code
22371	Marine sponge from a depth of 350 m near the Komandorskiye Islands	Russia	RUS
118949	Sponge	Russian Federation	RUS

Taxonmaps

69479

Global distribution of 16S sequence AF043742 (>99% sequence identity) for Pseudoalteromonas from Microbeatlas

Aquatic Samples	2792
Soil Samples	24
Animal Samples	384
Plant Samples	22
Total Samples	3222

Safety information

Risk assessment

@ref	Biosafety level	Biosafety level comment
22371	1	Risk group (German classification)
118949	1	Risk group (French classification)

Sequence information

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
22371	Alteromonas distincta 16S ribosomal RNA gene, partial sequence	AF043742	1458	77608
124043	Pseudoalteromonas distincta strain ATCC 700518 16S ribosomal RNA gene, partial sequence.	OQ625907	1090	77608
124043	Pseudoalteromonas distincta strain ATCC 700518 16S ribosomal RNA gene, partial sequence.	OQ625993	1293	77608
124043	Pseudoalteromonas distincta strain ATCC 700518 16S ribosomal RNA gene, partial sequence.	OQ626011	1312	77608

Genome sequences

@ref	Description	Accession	Assembly level	Database	NCBI tax ID
66792	Pseudoalteromonas distincta ATCC 700518	77608.3	wgs	PATRIC	77608
66792	Pseudoalteromonas distincta ATCC 700518	2627854061	draft	JGI IMG/M	77608
66792	Pseudoalteromonas distincta ATCC 700518	GCA_000814675	scaffold		77608

Genome-based predictions

Predictions

Predictions from Diaspora project based on:
Pseudoalteromonas distincta ATCC 700518
NCBI: GCA_000814675

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	99.30	no
125439	motility	BacteriaNetⓘ	yes	68.10	no
125439	gram_stain	BacteriaNetⓘ	negative	99.40	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.50	no

Predictions from Diaspora project based on:
Pseudoalteromonas distincta ATCC 700518
PATRIC: 77608.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	100.00	no
125438	anaerobic	anaerobicⓘ	no	96.42	no
125438	aerobic	aerobicⓘ	yes	77.17	no
125438	spore-forming	spore-formingⓘ	no	87.61	no
125438	thermophilic	thermophileⓘ	no	98.50	yes
125438	flagellated	motile2+ⓘ	yes	86.20	no

External links

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Genome-Based Classification of Strain 16-SW-7, a Marine Bacterium Capable of Converting B Red Blood Cells, as Pseudoalteromonas distincta and Proposal to Reclassify Pseudoalteromonas paragorgicola as a Later Heterotypic Synonym of Pseudoalteromonas distincta.	Nedashkovkaya OI, Kim SG, Balabanova LA, Zhukova NV, Son OM, Tekutyeva LA, Mikhailov VV	Front Microbiol	10.3389/fmicb.2021.809431	2022
Phylogeny	Assignment of Alteromonas elyakovii KMM 162T and five strains isolated from spot-wounded fronds of Laminaria japonica to Pseudoalteromonas elyakovii comb. nov. and the extended description of the species.	Sawabe T, Tanaka R, Iqbal MM, Tajima K, Ezura Y, Ivanova EP, Christen R	Int J Syst Evol Microbiol	10.1099/00207713-50-1-265	2000
Enzymology	Structure of an acidic polysaccharide from a marine bacterium Pseudoalteromonas distincta KMM 638 containing 5-acetamido-3,5,7,9-tetradeoxy-7-formamido-L-glycero-L-manno-nonulosonic acid.	Muldoon J, Shashkov AS, Senchenkova SN, Tomshich SV, Komandrova NA, Romanenko LA, Knirel YA, Savage AV	Carbohydr Res	10.1016/s0008-6215(00)00280-9	2001
Phylogeny	Reclassification of Alteromonas distincta Romanenko et al. 1995 as Pseudoalteromonas distincta comb. nov.	Ivanova EP, Chun J, Romanenko LA, Matte ME, Mikhailov VV, Frolova GM, Huq A, Colwell RR	Int J Syst Evol Microbiol	10.1099/00207713-50-1-141	2000
Phylogeny	Pseudoalteromonas arctica sp. nov., an aerobic, psychrotolerant, marine bacterium isolated from Spitzbergen.	Al Khudary R, Stosser NI, Qoura F, Antranikian G	Int J Syst Evol Microbiol	10.1099/ijs.0.64963-0	2008

Straininfo Link

@ref	Straininfo	Culture collections
89542	46242	ATCC 700518, DSM 12749, CIP 105340

Culture collection no.

Culture collection no.
DSM 12749
ATCC 700518
CIP 105340
KMM 638
VKM B-2136 D

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#22371	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 12749
#42037	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#89542	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID46242.1 )
#118949	Collection of Institut Pasteur ; Curators of the CIP; CIP 105340
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

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