Cite this strain

Marinospirillum megaterium (CIP 106459, JCM 10129, CGMCC 1.2728)

Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 138354

Type strain

Strain Designation H7

Culture col. no. CIP 106459 JCM 10129 CGMCC 1.2728 H7

NCBI tax ID(s) 64975

Special Collections JCM CIP

Links

Name and taxonomic classification

Marinospirillum megaterium H7 is a psychrophilic, Gram-negative, motile bacterium of the family Oceanospirillaceae.

Gram-negative motile rod-shaped psychrophilic 16S sequence Bacteria

SI-ID 69233

CIP 106459, JCM 10129

Last LPSN update 2024-12-09 10:49:47

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Oceanospirillales

Family Oceanospirillaceae

Genus Marinospirillum

Species Marinospirillum megaterium

Full scientific name Marinospirillum megaterium Satomi et al. 1998

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
39535	negative	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Growth	Composition
39535	MEDIUM 346 - for Marinospirillum megaterium		Distilled water make up to (1000.000 ml);Sodium chloride (30.000 g);Agar(2.000 g);Tryptone (17.000 g);Yeast nitrogen base (1.000 g);Soytone peptone (3.000 g)
39535	CIP Medium 346

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
39535	positive	growth	15	psychrophilic
39535	positive	growth	5-30
39535	negative	growth	37
39535	negative	growth	41
67770	positive	growth	20	psychrophilic

Physiology and metabolism

Observation

67770

Observationquinones: Q-8

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
39535	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
39535	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
39535	gamma-glutamyltransferase	-	2.3.2.2
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68382	trypsin	-	3.4.21.4	from API zym
39535	tryptophan deaminase	-
39535	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

Metabolite production

@ref	Chebi-ID	Metabolite	Production
39535	35581 ChEBI	indole

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
39535	17632 ChEBI	nitrate	-	reduction
39535	16301 ChEBI	nitrite	-	reduction

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
39535	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation

@ref	Geographic location	Country	Country ISO 3 Code	Continent
39535	Izu Islands, Tokyo	Japan	JPN	Asia
67770	Izu Islands	Japan	JPN	Asia
39535	Izu Islands, Tokyo	Japan	JPN	Asia

Taxonmaps

69479

Global distribution of 16S sequence AB006770 (>99% sequence identity) for Marinospirillum megaterium from Microbeatlas

Aquatic Samples	7
Soil Samples	20
Animal Samples	80
Plant Samples	8
Total Samples	115

Safety information

Risk assessment

@ref	Biosafety level	Biosafety level comment
39535	1	Risk group (French classification)

Sequence information

GC content

@ref	GC-content (mol%)	Method
67770	44.4	high performance liquid chromatography (HPLC)

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
67770	Marinospirillum megaterium gene for 16S rRNA, partial sequence	AB006770	1456		64975

Genome-based predictions

External links

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Marinospirillum gen. nov., with descriptions of Marinospirillum megaterium sp. nov., isolated from kusaya gravy, and transfer of Oceanospirillum minutulum to Marinospirillum minutulum comb. nov.	Satomi M, Kimura B, Hayashi M, Shouzen Y, Okuzumi M, Fujii T	Int J Syst Bacteriol	10.1099/00207713-48-4-1341	1998

Straininfo Link

@ref	Straininfo	Culture collections
95107	69233	CIP 106459, JCM 10129
95108	312132	CGMCC 1.2728

Culture collection no.

Culture collection no.
CIP 106459
JCM 10129
CGMCC 1.2728
H7

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#39535	Collection of Institut Pasteur ; Curators of the CIP; CIP 106459
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#95107	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID69233.1 )
#95108	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID312132.1 )

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