Cite this strain

Psychromicrobium lacuslunae (JCM 31143, KACC 19070, MCC 2780)

BacDive ID 159836

Type strain

Culture col. no. JCM 31143 KACC 19070 MCC 2780 MTCC 12460 IHBB 11108

NCBI tax ID(s) 1618207

Special Collections JCM Literature strains

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Psychromicrobium lacuslunae JCM 31143 is an aerobe, Gram-positive bacterium that was isolated from during bacterial diversity analysis from the subsurface water of Chandra Tal Lake.

Gram-positive aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 132058

JCM 31143

@ref 20215

Last LPSN update 2026-02-09 11:38:44

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Micrococcaceae

Genus Psychromicrobium

Species Psychromicrobium lacuslunae

Full scientific name Psychromicrobium lacuslunae Kiran et al. 2018

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Motility
66714	positive	1.1-1.25 µm	0.5-0.8 µm
125438

Colony morphology

@ref	Colony color	Incubation period	Medium used
66714	creamy	1 day	Trypticase Soy Agar (TSA)

Culture and growth conditions

Culture medium

@ref	Name	Growth
66714	tryptic soy agar (TSA)

Culture temp

@ref	Growth	Type	Temperature (°C)
66714	positive	optimum	28
66714	positive	growth	5-37
67770	positive	growth	28

Culture pH

@ref	Ability	Type	PH
66714	positive	optimum	7
66714	positive	growth	5-12

Physiology and metabolism

Oxygen tolerance

66714

Oxygen toleranceaerobe

Halophily

@ref	Salt	Growth	Tested relation	Concentration
66714	NaCl	positive	growth	0-8 %(w/v)
66714	NaCl	positive	optimum	1 %(w/v)

Observation

67770

Observationquinones: MK-9(H₂), MK-10(H₂)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
66714	16651 ChEBI	(S)-lactate	+	carbon source
66714		1 % sodium lactate	+	carbon source
66714	16763 ChEBI	2-oxobutanoate	-	carbon source
66714	16810 ChEBI	2-oxoglutarate	-	carbon source
66714	37054 ChEBI	3-hydroxybutyrate	+	carbon source
66714	73918 ChEBI	3-O-methyl-D-glucose	-	carbon source
66714	18101 ChEBI	4-hydroxyphenylacetic acid	+	carbon source
66714	16411 ChEBI	acetic acid	+	carbon source
66714	13705 ChEBI	acetoacetate	+	carbon source
66714	17128 ChEBI	adipate	+	assimilation
66714	17925 ChEBI	alpha-D-glucose	+	carbon source
66714		alpha-hydroxybutyrate	+	carbon source
66714	29016 ChEBI	arginine	+	hydrolysis
66714	161680 ChEBI	aztreonam	+	carbon source
66714	73706 ChEBI	bromosuccinate	+	carbon source
66714		casein	+	hydrolysis
66714	17057 ChEBI	cellobiose	-	carbon source
66714	62968 ChEBI	cellulose	-	hydrolysis
66714	53258 ChEBI	citric acid	+	carbon source
66714	18333 ChEBI	D-arabitol	+	carbon source
66714	29990 ChEBI	D-aspartate	-	carbon source
66714	15824 ChEBI	D-fructose	+	carbon source
66714	78697 ChEBI	D-fructose 6-phosphate	-	carbon source
66714	28847 ChEBI	D-fucose	-	carbon source
66714	12936 ChEBI	D-galactose	+	carbon source
66714	18024 ChEBI	D-galacturonic acid	-	carbon source
66714	30612 ChEBI	D-glucarate	-	carbon source
66714	8391 ChEBI	D-gluconate	+	assimilation
66714	17634 ChEBI	D-glucose	-	assimilation
66714	14314 ChEBI	D-glucose 6-phosphate	+	carbon source
66714	15748 ChEBI	D-glucuronate	-	carbon source
66714	15748 ChEBI	D-glucuronate	+	carbon source
66714	15588 ChEBI	D-malate	+	carbon source
66714	16899 ChEBI	D-mannitol	-	assimilation
66714	16899 ChEBI	D-mannitol	+	carbon source
66714	16024 ChEBI	D-mannose	-	assimilation
66714	16024 ChEBI	D-mannose	+	carbon source
66714	16523 ChEBI	D-serine	-	carbon source
66714	17924 ChEBI	D-sorbitol	+	carbon source
66714	16551 ChEBI	D-trehalose	+	carbon source
66714	27689 ChEBI	decanoate	-	assimilation
66714	23652 ChEBI	dextrin	+	carbon source
66714	4853 ChEBI	esculin	+	hydrolysis
66714	15740 ChEBI	formate	+	carbon source
66714	71321 ChEBI	fusidate	-	carbon source
66714	16537 ChEBI	galactarate	-	carbon source
66714	16865 ChEBI	gamma-aminobutyric acid	-	carbon source
66714	5291 ChEBI	gelatin	+	carbon source
66714	5291 ChEBI	gelatin	+	hydrolysis
66714	28066 ChEBI	gentiobiose	+	carbon source
66714	32323 ChEBI	glucuronamide	-	carbon source
66714	17754 ChEBI	glycerol	+	carbon source
66714	70744 ChEBI	glycine-proline	+	carbon source
66714	32735 ChEBI	guanidinium chloride	+	assimilation
66714	17596 ChEBI	inosine	+	carbon source
66714	16977 ChEBI	L-alanine	+	carbon source
66714	30849 ChEBI	L-arabinose	-	assimilation
66714	16467 ChEBI	L-arginine	+	carbon source
66714	29991 ChEBI	L-aspartate	-	carbon source
66714	18287 ChEBI	L-fucose	+	carbon source
66714	17464 ChEBI	L-galactonic acid gamma-lactone	-	carbon source
66714	29985 ChEBI	L-glutamate	+	carbon source
66714	15971 ChEBI	L-histidine	+	carbon source
66714	15589 ChEBI	L-malate	+	carbon source
66714	18183 ChEBI	L-pyroglutamic acid	+	carbon source
66714	62345 ChEBI	L-rhamnose	-	carbon source
66714	17115 ChEBI	L-serine	+	carbon source
66714	17716 ChEBI	lactose	+	carbon source
66714	48607 ChEBI	lithium chloride	+	carbon source
66714	25115 ChEBI	malate	-	assimilation
66714	17306 ChEBI	maltose	-	assimilation
66714	17306 ChEBI	maltose	+	carbon source
66714	28053 ChEBI	melibiose	+	carbon source
66714	74611 ChEBI	methyl (R)-lactate	+	carbon source
66714	320055 ChEBI	methyl beta-D-glucopyranoside	-	carbon source
66714	51850 ChEBI	methyl pyruvate	-	carbon source
66714	17268 ChEBI	myo-inositol	+	carbon source
66714	63153 ChEBI	N-acetyl-D-mannosamine	-	carbon source
66714	28800 ChEBI	N-acetylgalactosamine	+	carbon source
66714	59640 ChEBI	N-acetylglucosamine	-	assimilation
66714	59640 ChEBI	N-acetylglucosamine	+	carbon source
66714	35418 ChEBI	n-acetylneuraminate	-	carbon source
66714	100147 ChEBI	nalidixic acid	+	carbon source
66714	17632 ChEBI	nitrate	-	reduction
66714	17309 ChEBI	pectin	+	carbon source
66714	18401 ChEBI	phenylacetate	+	assimilation
66714	32032 ChEBI	potassium gluconate	+	assimilation
66714	75248 ChEBI	potassium tellurite	-	carbon source
66714	17272 ChEBI	propionate	+	carbon source
66714	26490 ChEBI	quinate	-	carbon source
66714	16634 ChEBI	raffinose	+	carbon source
66714	17814 ChEBI	salicin	+	carbon source
66714	75229 ChEBI	sodium bromate	+	carbon source
66714	64103 ChEBI	sodium butyrate	+	carbon source
66714	53258 ChEBI	sodium citrate	+	assimilation
66714	17164 ChEBI	stachyose	-	carbon source
66714	28017 ChEBI	starch	-	hydrolysis
66714	17992 ChEBI	sucrose	+	carbon source
66714	75198 ChEBI	tetrazolium blue	+	assimilation
66714	32528 ChEBI	turanose	+	carbon source
66714	53424 ChEBI	tween 20	+	hydrolysis
66714	53423 ChEBI	tween 40	+	carbon source
66714	53423 ChEBI	tween 40	+	hydrolysis
66714	53426 ChEBI	tween 80	-	hydrolysis

Metabolite production

@ref	Chebi-ID	Metabolite	Production
66714	16411 ChEBI	indole-3-acetic acid

Enzymes

@ref	Value	Activity	Ec
66714	acid phosphatase	+	3.1.3.2
66714	alkaline phosphatase	-	3.1.3.1
66714	alpha-chymotrypsin	-	3.4.21.1
66714	alpha-fucosidase	-	3.2.1.51
66714	alpha-galactosidase	-	3.2.1.22
66714	alpha-glucosidase	+	3.2.1.20
66714	alpha-mannosidase	+	3.2.1.24
66714	beta-galactosidase	+	3.2.1.23
66714	beta-glucosidase	+	3.2.1.21
66714	beta-glucuronidase	-	3.2.1.31
66714	catalase	+	1.11.1.6
66714	cystine arylamidase	-	3.4.11.3
66714	cytochrome oxidase	-	1.9.3.1
66714	esterase (C 4)	+
66714	esterase Lipase (C 8)	+
66714	leucine arylamidase	+	3.4.11.1
66714	lipase (C 14)	-
66714	lysine decarboxylase	+	4.1.1.18
66714	N-acetyl-beta-glucosaminidase	-	3.2.1.52
66714	naphthol-AS-BI-phosphohydrolase	+
66714	ornithine decarboxylase	+	4.1.1.17
66714	trypsin	-	3.4.21.4
66714	urease	-	3.5.1.5
66714	valine arylamidase	-

Fatty acid profile

Metadata FA analysis

@ref

66714

Fatty acid	Percentage
C15:0 anteiso	17.7
C16:0 iso	14.4
C17:0 anteiso	10.1
C16:1 w5c	9.6
C15:1 w5c	7.9
C16:0	7.6
C13:0 anteiso	5.3
C18:0	5.1
C17:0 iso 3OH	3.3
C18:1 w7c/ C18:1 w6c	2.8
C15:0 iso	2.2
C19:0 iso	2.2
C16:1 OH	2.1
C17:0 2OH	1.5
C16:0 iso 3OH	0.9
C19:0 cyclo w8c	0.9

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Lake (large)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude	Enrichment culture	Enrichment culture composition	Enrichment culture duration	Enrichment culture temperature	Isolation procedure
66714	during bacterial diversity analysis from the subsurface water of Chandra Tal Lake	Chandra Tal Lake in the Spiti valley of the state of Himachal Pradesh	India	IND	Asia	32.4349	77.6375 32.4349/77.6375	tryptone soya agar	actidione (50 µg ml-1)	15 days	28	isolated during bacterial diversity analysis from the subsurface water of Chandra Tal Lake situated in the cold desert of the Indian trans-Himalayas. Water samples were collected in 2 l sterile Nalgene bottles and stored at 4°C in a refrigerator. The bacterial isolations were made by serial dilution of a 10 ml water sample in 90 ml 0.85 % sterile saline (NaCl 0.85 g l-1), spread plating of 100 µl of a 10-3 dilution on tryptone soya agar (TSA) supplemented with actidione (50 µg ml-1), and incubation at 28 °C for 15 days.
67770	Shallow undersurface water from Chandra Tal Lake	Lahaul-Spiti, Himachal Pradesh	India	IND	Asia

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM95057v1 assembly for Psychromicrobium lacuslunae IHBB 11108	complete	GCA_000950575	1618207.4	2654588025	1618207	98.4

Genome browser: GCA_000950575

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
66714	Psychromicrobium lacuslunae strain IHBB 11108 16S ribosomal RNA gene, partial sequence	KU921532	1493		1618207
124043	Psychromicrobium lacuslunae strain IHBB 11108 16S ribosomal RNA gene, partial sequence.	KP745130	1481		1618207

GC content

@ref	GC-content (mol%)	Method
66714	59	genome sequence analysis
67770	58.97	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Diaspora project based on:
Arthrobacter sp. IHBB 11108
PATRIC: 1618207.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	89.10	yes
125438	anaerobic	anaerobicⓘ	no	98.35	no
125438	spore-forming	spore-formingⓘ	no	72.57	no
125438	aerobic	aerobicⓘ	yes	86.73	no
125438	thermophilic	thermophileⓘ	no	98.30	yes
125438	flagellated	motile2+ⓘ	no	92.00	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	The landscape of bacterial presence in tumor and adjacent normal tissue across 9 major cancer types using TCGA exome sequencing.	Rodriguez RM, Hernandez BY, Menor M, Deng Y, Khadka VS.	Comput Struct Biotechnol J	10.1016/j.csbj.2020.03.003	2020
	Psychromicrobium Xiongbiense sp. nov., an Actinobacterium Isolated from Forest Soil.	Zhu L, Wang YC, Li LL, Huang XM, Zhu M, Gao X, Cai J.	Curr Microbiol	10.1007/s00284-024-03629-5	2024
Phylogeny	Psychromicrobium lacuslunae sp. nov., isolated from a high altitude lake.	Kiran S, Schumann P, Busse HJ, Sproer C, Rana A, Pal M, Korpole S, Tewari R, Gulati A	Int J Syst Evol Microbiol	10.1099/ijsem.0.002997	2018

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66714	Shashi Kiran, Peter Schumann, Hans-Jürgen Busse, Cathrin Spröer, Aditi Rana, Mohinder Pal, Suresh Korpole, Rupinder Tewari, Arvind Gulati: Psychromicrobium lacuslunae sp. nov., isolated from a high altitude lake. IJSEM 68: 3416 - 3423 2018 ( DOI 10.1099/ijsem.0.002997 , PubMed 30204584 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Psychromicrobium lacuslunae (JCM 31143, KACC 19070, MCC 2780)

Name and taxonomic classification