Cite this strain

Clostridium tetanomorphum (DSM 4474, ATCC 49273, NCTC 543)

Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2727

Type strain

Strain Designation 259 E.III.

Culture col. no. DSM 4474 ATCC 49273 NCTC 543 259 E.III.

NCBI tax ID(s) 1553

Special Collections DSMZ

Links

Name and taxonomic classification

Clostridium tetanomorphum 259 E.III. is an anaerobe, mesophilic bacterium that produces alcohol and was isolated from septic wound.

alcohol production anaerobe mesophilic genome sequence 16S sequence Bacteria

SI-ID 42396

ATCC 49273, NCTC 543, DSM 4474, BCRC 14534, CCRC 14534

Last LPSN update 2024-12-09 10:43:26

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Eubacteriales

Family Clostridiaceae

Genus Clostridium

Species Clostridium tetanomorphum

Full scientific name Clostridium tetanomorphum (ex Bulloch et al. 1919) Wilde et al. 1989

BacDive ID	Other strains from **Clostridium tetanomorphum** (3)	Type strain
2725	C. tetanomorphum H1, DSM 528, ATCC 15920
2726	C. tetanomorphum 297 VII, DSM 665, NCTC 288
134685	C. tetanomorphum 1772C, CIP 108179

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
1687	WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339)		Medium recipe at MediaDive	Name: WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339) Composition: dehydrated Wilkins-Chalgren medium 33.0 g/l L-Cysteine HCl 0.3 g/l Sodium resazurin 0.0005 g/l Distilled water
1687	CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)		Medium recipe at MediaDive	Name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l K2HPO4 5.0 g/l Yeast extract 5.0 g/l D-Glucose 4.0 g/l Starch 1.0 g/l Maltose 1.0 g/l Cellobiose 1.0 g/l L-Cysteine HCl 0.5 g/l Ethanol 0.19 g/l Vitamin K3 0.05 g/l Hemin 0.005 g/l Sodium resazurin 0.0005 g/l Vitamin K1 NaOH Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
1687	positive	growth	37	mesophilic

Physiology and metabolism

Compound production

1687

Compoundn butanol

Oxygen tolerance

@ref	Oxygen tolerance
1687	anaerobe
125438	anaerobe
125439	anaerobe

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Infection	#Disease
#Host Body-Site	#Other

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
1687	septic wound	United Kingdom	GBR	Europe

Taxonmaps

69479

Global distribution of 16S sequence DQ241819 (>99% sequence identity) for Clostridium tetanomorphum subclade from Microbeatlas

Aquatic Samples	850
Soil Samples	1355
Animal Samples	6449
Plant Samples	176
Total Samples	8830

Safety information

Risk assessment

@ref	Pathogenicity human	Biosafety level	Biosafety level comment
1687	yes, in single cases	1	Risk group (German classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Clostridium tetanomorphum strain DSM 4474 16S ribosomal RNA gene, partial sequence	DQ911261	320		1553
1687	Clostridium tetanomorphum strain DSM 4474 16S ribosomal RNA gene, partial sequence	DQ241819	1407		1553

Genome sequences

@ref	Description	Accession	Assembly level	Database	NCBI tax ID
66792	Clostridium tetanomorphum DSM 4474	GCA_017873215	contig		1553
66792	Clostridium tetanomorphum strain DSM 4474	1553.15	wgs	PATRIC	1553
66792	Clostridium tetanomorphum DSM 4474	2913338184	draft	JGI IMG/M	1553

Genome-based predictions

Predictions

Predictions from Diaspora project based on:
Clostridium tetanomorphum DSM 4474
NCBI: GCA_017873215

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	76.90	no
125439	motility	BacteriaNetⓘ	yes	76.20	no
125439	gram_stain	BacteriaNetⓘ	positive	76.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	98.50	no

Predictions from Diaspora project based on:
Clostridium tetanomorphum strain DSM 4474
PATRIC: 1553.15

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	50.73	no
125438	anaerobic	anaerobicⓘ	yes	90.11	no
125438	spore-forming	spore-formingⓘ	yes	76.80	no
125438	thermophilic	thermophileⓘ	no	88.66	yes
125438	aerobic	aerobicⓘ	no	96.64	yes
125438	flagellated	motile2+ⓘ	yes	75.26	no

External links

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Clostridium beihaiense sp. nov., an anaerobic bacterium isolated from activated sludge.	Dong Y, Liu Y, Chen N, Zhong Y, Liu L, Xie Q	Int J Syst Evol Microbiol	10.1099/ijsem.0.002885	2018
Phylogeny	Clostridium liquoris sp. nov., isolated from a fermentation pit used for the production of Chinese strong-flavoured liquor.	Yin Q, Tao Y, Zhu X, Zhou Y, He X, Cheng L, Huang Y, Li D	Int J Syst Evol Microbiol	10.1099/ijsem.0.000787	2015
Phylogeny	Clostridium lundense sp. nov., a novel anaerobic lipolytic bacterium isolated from bovine rumen.	Cirne DG, Delgado OD, Marichamy S, Mattiasson B	Int J Syst Evol Microbiol	10.1099/ijs.0.63730-0	2006

Straininfo Link

@ref	Straininfo	Culture collections
72255	42396	ATCC 49273, NCTC 543, DSM 4474, BCRC 14534, CCRC 14534

Culture collection no.

Culture collection no.
DSM 4474
ATCC 49273
NCTC 543
259 E.III.

References

#1687	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 4474
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#72255	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID42396.1 )
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

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