Cite this strain

Aeropyrum pernix (DSM 11879, JCM 9820, K1)

Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 4195

Type strain

Strain Designation K1

Culture col. no. DSM 11879 JCM 9820 K1 ATCC 700893 NBRC 100138

NCBI tax ID(s) 56636

Special Collections DSMZ JCM Literature strains

Links

Name and taxonomic classification

Aeropyrum pernix K1 is an aerobe, heterotroph, hyperthermophilic archaeon that forms irregular colonies and was isolated from marine solfataric vent.

colony-forming coccus-shaped motile Gram-negative hyperthermophilic heterotroph aerobe Archaea 16S sequence genome sequence

SI-ID 44195

ATCC 700893, JCM 9820, DSM 11879, NBRC 100138

Last LPSN update 2024-12-09 10:43:36

Domain Archaea

Phylum Thermoproteota

Class Thermoprotei

Order Acidilobales

Family Acidilobaceae

Genus Aeropyrum

Species Aeropyrum pernix

Full scientific name Aeropyrum pernix Sako et al. 1996

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell shape	Motility
23222	negative	0.8-1.0 µm	coccus-shaped

Colony morphology

@ref	Colony color	Colony shape
23222	brown	irregular

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
4521	BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)		Medium recipe at MediaDive	Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514; with strain-specific modifications) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l Na2S2O3 x 5 H2O 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
4521	positive	growth	90	hyperthermophilic
23222	positive	growth	70-100	hyperthermophilic
23222	negative	growth	68
23222	negative	growth	102
23222	positive	optimum	90-95	hyperthermophilic
67770	positive	growth	90	hyperthermophilic

Culture pH

@ref	Ability	Type	PH
23222	positive	optimum	7.0
23222	positive	growth	5-9

Physiology and metabolism

Observation

67770

Observationquinones: DMK-6(H_n), MTK-6(H_n)

Spore formation

@ref	Spore formation	Confidence
125438		91.512

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23222	16094 ChEBI	thiosulfate	+	growth

Nutrition type

23222

Typeheterotroph

Oxygen tolerance

@ref	Oxygen tolerance
23222	aerobe
125439	anaerobe

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Condition	#Sulfuric
#Condition	#Thermophilic (>45°C)
#Environmental	#Aquatic
#Environmental	#Terrestrial

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
4521	marine solfataric vent	Kodakara-Jima Island	Japan	JPN	Asia
23222	hot sedimentary materials and venting waters at a coastal solfataric vent
67770	Coastal solfataric vent at Kodakara-Jima Island		Japan	JPN	Asia

Safety information

Risk assessment

@ref	Biosafety level	Biosafety level comment
4521	1	Risk group (German classification) According to TRBA 466

Sequence information

GC content

@ref	GC-content (mol%)	Method
4521	67	high performance liquid chromatography (HPLC)
23222	67.0	high performance liquid chromatography (HPLC)

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
4521	Aeropyrum pernix gene for 16S rRNA, partial sequence	D83259	1444		56636
67770	Aeropyrum pernix genes for 16S rRNA, 23S rRNA and rRNA intron-encoded homing endonuclease, complete cds	AB008745	10777		56636

Genome sequences

@ref	Description	Accession	Assembly level	Database	NCBI tax ID
66792	Aeropyrum pernix K1	GCA_000011125	complete		272557
66792	Aeropyrum pernix K1	2923402010	complete	JGI IMG/M	272557
66792	Aeropyrum pernix K1	272557.25	complete	PATRIC	272557

Genome-based predictions

Predictions

Predictions from Diaspora project based on:
Aeropyrum pernix K1
NCBI: GCA_000011125

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	67.10	no
125439	motility	BacteriaNetⓘ	no	51.50	no
125439	gram_stain	BacteriaNetⓘ	positive	69.60	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	99.60	no

Predictions from Diaspora project based on:
Aeropyrum pernix K1 K1
NCBI: GCA_000011125.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	78.25	yes
125438	anaerobic	anaerobicⓘ	yes	50.35	yes
125438	aerobic	aerobicⓘ	no	56.33	no
125438	spore-forming	spore-formingⓘ	no	91.51	no
125438	thermophilic	thermophileⓘ	yes	82.40	yes
125438	flagellated	motile2+ⓘ	no	84.50	no

External links

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Kinetic study of sn-glycerol-1-phosphate dehydrogenase from the aerobic hyperthermophilic archaeon, Aeropyrum pernix K1.	Han JS, Kosugi Y, Ishida H, Ishikawa K	Eur J Biochem	10.1046/j.0014-2956.2001.02731.x	2002
Enzymology	Glutamate dehydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum pernix K1: enzymatic characterization, identification of the encoding gene, and phylogenetic implications.	Bhuiya MW, Sakuraba H, Kujo C, Nunoura-Kominato N, Kawarabayasi Y, Kikuchi H, Ohshima T	Extremophiles	10.1007/s007920070002	2000
Enzymology	Purification and characterization of an intracellular heat-stable proteinase (pernilase) from the marine hyperthermophilic archaeon Aeropyrum pernix K1.	Chavez Croocker P, Sako Y, Uchida A	Extremophiles	10.1007/s007920050093	1999
Enzymology	An extremely heat-stable extracellular proteinase (aeropyrolysin) from the hyperthermophilic archaeon Aeropyrum pernix K1.	Sako Y, Croocker PC, Ishida Y	FEBS Lett	10.1016/s0014-5793(97)01153-8	1997
Phylogeny	Aeropyrum pernix gen. nov., sp. nov., a novel aerobic hyperthermophilic archaeon growing at temperatures up to 100 degrees C.	Sako Y, Nomura N, Uchida A, Ishida Y, Morii H, Koga Y, Hoaki T, Maruyama T	Int J Syst Bacteriol	10.1099/00207713-46-4-1070	1996

Straininfo Link

@ref	Straininfo	Culture collections
73695	44195	ATCC 700893, JCM 9820, DSM 11879, NBRC 100138

Culture collection no.

Culture collection no.
DSM 11879
JCM 9820
K1
ATCC 700893
NBRC 100138

References

#4521	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 11879
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#23222	YOSHIHIKO SAKO, NORIMICHI NOMURA, ARITSUNE UCHIDA, YUZABURO ISHIDA, HIROYUKI MORII, YOSUKE KOGA, TOSHIHIRO HOAKI, TADASHI MARUYAMA: Aeropyrum pernix gen. nov., sp. nov., a Novel Aerobic Hyperthermophilic Archaeon Growing at Temperatures up to 100°C. IJSEM 46: 1070 - 1077 1996 ( DOI 10.1099/00207713-46-4-1070 , PubMed 8863437 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#73695	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID44195.1 )
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

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