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Bacterial strain

Klebsiella pneumoniae (CCUG 58866, DSM 789, ATCC 4352)

BacDive ID 4969
Type strain
Culture col. no. CCUG 58866 DSM 789 ATCC 4352 NCIB 10341 WDCM 00192 NCIMB 10341 NBRC 13277 IFO 13277 IAM 12015 CIP 104216 JCM 20348 CECT 8453 NCTC 13635 8 more
NCBI tax ID(s) 573
Special Collections DSMZ CCUG JCM CIP
Links

Morphology

Cell morphology

@refGram stainCell shapeMotility
121885negativerod-shaped
125439negative
125438negative

Culture and growth conditions

Culture medium

@refNameGrowthMedium linkComposition
447COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)Medium recipe at MediaDive MediaDiveName: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
41831MEDIUM 3 - Columbia agarColumbia agar (39.000 g);distilled water (1000.000 ml)
447TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)Medium recipe at MediaDive MediaDiveName: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
121885CIP Medium 3Medium recipe at CIP
121885CIP Medium 72Medium recipe at CIP

Culture temp

@refGrowthTypeTemperature (°C)Range
447positivegrowth37mesophilic
41831positivegrowth30mesophilic
61767positivegrowth30mesophilic
67770positivegrowth37mesophilic
121885positivegrowth30-41
121885negativegrowth5
121885negativegrowth10

Physiology and metabolism

Spore formation

@refSpore formationConfidence
12543997.9

Enzymes

@refValueActivityEc
68382acid phosphatase+3.1.3.2
121885alcohol dehydrogenase-1.1.1.1
68382alkaline phosphatase+3.1.3.1
68382alpha-chymotrypsin-3.4.21.1
68382alpha-fucosidase-3.2.1.51
68382alpha-galactosidase+3.2.1.22
68374alpha-galactosidase+3.2.1.22
68382alpha-glucosidase+3.2.1.20
68374alpha-glucosidase-3.2.1.20
68374alpha-maltosidase-
68382alpha-mannosidase-3.2.1.24
68374arginine dihydrolase-3.5.3.6
68368arginine dihydrolase-3.5.3.6
68382beta-galactosidase+3.2.1.23
121885beta-galactosidase+3.2.1.23
68374beta-galactosidase+3.2.1.23
68368beta-galactosidase+3.2.1.23
68382beta-glucosidase+3.2.1.21
68374beta-glucosidase+3.2.1.21
68382beta-glucuronidase-3.2.1.31
68374beta-glucuronidase-3.2.1.31
121885catalase+1.11.1.6
68382cystine arylamidase-3.4.11.3
68368cytochrome oxidase-1.9.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68368gelatinase-
68374L-aspartate arylamidase-3.4.11.21
68382leucine arylamidase+3.4.11.1
68374lipase-
68382lipase (C 14)-
121885lysine decarboxylase+4.1.1.18
68374lysine decarboxylase+4.1.1.18
68368lysine decarboxylase+4.1.1.18
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68374N-acetyl-beta-glucosaminidase-3.2.1.52
68382naphthol-AS-BI-phosphohydrolase+
121885ornithine decarboxylase-4.1.1.17
68374ornithine decarboxylase-4.1.1.17
68368ornithine decarboxylase-4.1.1.17
121885oxidase-
68382trypsin-3.4.21.4
68368tryptophan deaminase-4.1.99.1
121885urease+3.5.1.5
68374urease-3.5.1.5
68368urease+3.5.1.5
68382valine arylamidase-

Metabolite production

@refChebi-IDMetaboliteProduction
6836815688 ChEBIacetoin
6836816136 ChEBIhydrogen sulfide
12188535581 ChEBIindole
6837435581 ChEBIindole
6836835581 ChEBIindole

Metabolite tests

@refChebi-IDMetaboliteVoges-proskauer-test
6836815688 ChEBIacetoin-
6837435581 ChEBIindole
6836835581 ChEBIindole

Metabolite utilization

@refChebi-IDMetaboliteUtilization activityKind of utilization tested
6836827613 ChEBIamygdalin+fermentation
6837429016 ChEBIarginine-hydrolysis
6836829016 ChEBIarginine-hydrolysis
6837417057 ChEBIcellobiose+builds acid from
6837418333 ChEBID-arabitol+builds acid from
6837418024 ChEBID-galacturonic acid+builds acid from
6837417634 ChEBID-glucose+builds acid from
6836817634 ChEBID-glucose+fermentation
6837416899 ChEBID-mannitol+builds acid from
6836816899 ChEBID-mannitol+fermentation
683685291 ChEBIgelatin-hydrolysis
12188517234 ChEBIglucose+fermentation
12188517234 ChEBIglucose+degradation
6837430849 ChEBIL-arabinose+builds acid from
6836830849 ChEBIL-arabinose+fermentation
6837418403 ChEBIL-arabitol-builds acid from
6837462345 ChEBIL-rhamnose+builds acid from
6836862345 ChEBIL-rhamnose+fermentation
12188517716 ChEBIlactose+fermentation
6837425094 ChEBIlysine+degradation
6836825094 ChEBIlysine+degradation
6837415792 ChEBImalonate-assimilation
6837417306 ChEBImaltose+builds acid from
12188529864 ChEBImannitol+fermentation
6836828053 ChEBImelibiose+fermentation
6837417268 ChEBImyo-inositol-builds acid from
6836817268 ChEBImyo-inositol-fermentation
12188517632 ChEBInitrate+reduction
12188516301 ChEBInitrite+reduction
6837418257 ChEBIornithine-degradation
6836818257 ChEBIornithine-degradation
6837418394 ChEBIpalatinose+builds acid from
68374Potassium 5-ketogluconate-builds acid from
6837415963 ChEBIribitol+builds acid from
121885132112 ChEBIsodium thiosulfate-builds gas from
6837430911 ChEBIsorbitol+builds acid from
6836830911 ChEBIsorbitol+fermentation
6837417992 ChEBIsucrose+builds acid from
6836817992 ChEBIsucrose+fermentation
6837427082 ChEBItrehalose+builds acid from
6837427897 ChEBItryptophan-energy source
6836827897 ChEBItryptophan-energy source
6837416199 ChEBIurea-hydrolysis
6836816199 ChEBIurea+hydrolysis

Oxygen tolerance

@refOxygen tolerance
61767aerobe
121885facultative anaerobe
125439aerobe

Antibiotic susceptibility testing

  Test 1 Test 2 Test 3
@ref 447 447 447
Medium Mueller-Hinton Agar Mueller-Hinton Agar Mueller-Hinton Agar
Incubation temperature 37 30 30
Incubation time 1
Oxygen condition aerob aerob aerob
Manual_annotation 1 1 1
Inhibition zone diameter in mm Inhibition zone diameter in mm Inhibition zone diameter in mm
Penicillin G 0 0 0
Oxacillin 6 8 8
Ampicillin 8 6 6
Ticarcillin 12 10 10
Mezlocillin 22 20 20
Cefalotin 32 30 30
Cefazolin 30 28-30 28-30
Cefotaxime 42 40 40
Aztreonam 38 38 38
Imipenem 32 30-32 30-32
Tetracycline 32 32 32
Chloramphenicol 36-38 38 38
Gentamycin 22 20 20
Amikacin 24 22 22
Vancomycin 6 0 0
Erythromycin 18-20 20-22 20-22
Lincomycin 10-12 10 10
Ofloxacin 40 40 40
Norfloxacin 40 38-40 38-40
Colistin 14 14 14
Pipemidic acid 32 28 28
Nitrofurantoin 24 22 22
Bacitracin 0 0 0
Polymyxin b 16 16 16
Kanamycin 24 22 22
Neomycin 18-20 18 18
Doxycycline 30 30-32 30-32
Ceftriaxone 38 36 36
Clindamycin 16 20 20
Fosfomycin 24 24 24
Moxifloxacin 40 38-40 38-40
Linezolid 26 22 22
Nystatin 0 0 0
Quinupristin/dalfopristin 12 14 14
Teicoplanin 0 0 0
Piperacillin/tazobactam 32 30 30

API biotype100

@ref ControlQ D-glucose as carbon sourceGLU D-fructose as carbon sourceFRU D-galactose as carbon sourceGAL D-trehalose as carbon sourceTRE D-mannose as carbon sourceMNE L-sorbose as carbon sourceSBE D-melibiose as carbon sourceMEL sucrose as carbon sourceSAC D-raffinose as carbon sourceRAF maltotriose as carbon sourceMTE maltose as carbon sourceMAL lactose as carbon sourceLAC lactulose as carbon sourceLTE 1-O-methyl-beta-galactopyranoside as carbon sourceMbGa 1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa D-cellobiose as carbon sourceCEL gentiobiose as carbon sourceGEN 1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu esculin as carbon sourceESC D-ribose as carbon sourceRIB L-arabinose as carbon sourceARA D-xylose as carbon sourceXYL palatinose as carbon sourcePLE L-rhamnose as carbon sourceRHA L-fucose as carbon sourceFUC D-melezitose as carbon sourceMLZ D-arabitol as carbon sourceDARL L-arabitol as carbon sourceLARL xylitol as carbon sourceXLT dulcitol as carbon sourceDUL D-tagatose as carbon sourceTAG glycerol as carbon sourceGLY myo-inositol as carbon sourceINO D-mannitol as carbon sourceMAN maltitol as carbon sourceMTL D-turanose as carbon sourceTUR D-sorbitol as carbon sourceSOR adonitol as carbon sourceADO hydroxyquinoline-beta-glucuronide as carbon sourceHBG D-lyxose as carbon sourceLYX i-erythritol as carbon sourceERY 1-O-methyl-alpha-D-glucoside as carbon sourceMDG 3-O-methyl-D-glucose as carbon source3MDG D-saccharate as carbon sourceSAT mucate as carbon sourceMUC L-tartrate as carbon sourceLTAT D-tartrate as carbon sourceDTAT meso-tartrate as carbon sourceMTAT D-malate as carbon sourceDMLT L-malate as carbon sourceLMLT cis-aconitate as carbon sourceCATE trans-aconitate as carbon sourceTATE tricarballylate as carbon sourceTTE citrate as carbon sourceCIT D-glucuronate as carbon sourceGRT D-galacturonate as carbon sourceGAT 2-ketogluconate as carbon source2KG 5-ketogluconate as carbon source5KG tryptophan as carbon sourceTRY N-acetyl-D-glucosamine as carbon sourceNAG D-gluconate as carbon sourceGNT phenylacetate as carbon sourcePAC protocatechuate as carbon sourcePAT 4-hydroxybenzoate as carbon sourcepOBE quinate as carbon sourceQAT gentisate as carbon sourceGTE 3-hydroxybenzoate as carbon sourcemOBE benzoate as carbon sourceBAT 3-phenylpropionate as carbon sourcePPAT m-coumarate as carbon sourceCMT trigonelline as carbon sourceTGE betaine as carbon sourceBET putrescine as carbon sourcePCE 4-aminobutyrate as carbon sourceABT histamine as carbon sourceHIN DL-lactate as carbon sourceLAT caprate as carbon sourceCAP caprylate as carbon sourceCYT L-histidine as carbon sourceHIS succinate as carbon sourceSUC fumarate as carbon sourceFUM glutarate as carbon sourceGRE DL-glycerate as carbon sourceGYT 5-aminovalerate as carbon sourceAVT ethanolamine as carbon sourceETN tryptamine as carbon sourceTTN D-glucosamine as carbon sourceGLN itaconate as carbon sourceITA 3-hydroxybutyrate as carbon source3OBU L-aspartate as carbon sourceAPT L-glutamate as carbon sourceGTT L-proline as carbon sourcePRO D-alanine as carbon sourceDALA L-alanine as carbon sourceLALA L-serine as carbon sourceSER malonate as carbon sourceMNT propionate as carbon sourcePROP L-tyrosine as carbon sourceTYR 2-ketoglutarate as carbon source2KT
121885 not determinedn.d. + + + + + + + + + + + + + + + + + + + + + + + + + - + - + - - + + + + + + + - - - + - + + + - + + + + + - + + + + - - + + + + + + - - + - + - - - + - + - - - + + - + + + - + - + + + + + + + - - - -

Isolation, sampling and environmental information

Isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Bovinae (Cow, Cattle)
#Host Body Product#Fluids#Milk

Isolation

@refSample type
447cow's milk
61767Milk,cow
67770Cow's milk
121885Food, Cow, milk

Safety information

Risk assessment

@refBiosafety levelBiosafety level comment
4472Risk group (German classification)
According to TRBA 466
1218852Risk group (French classification)
According to TRBA 466

Sequence information

16S sequences

@refDescriptionAccessionLengthDatabaseNCBI tax ID
124043Klebsiella pneumoniae gene for 16S rRNA, partial sequence, strain: NBRC 13277.AB6803921465

573  
124043Klebsiella pneumoniae subsp. pneumoniae JCM 20348 gene for 16S rRNA, partial sequence.LC6548891462

72407  

Genome sequences

@refDescriptionAccessionAssembly levelDatabaseNCBI tax ID
66792Klebsiella pneumoniae ATCC 4352GCA_010374885scaffold

573  
66792Klebsiella pneumoniae strain ATCC 4352573.30826wgs
PATRIC
573  

Genome-based predictions

Predictions

Predictions from Diaspora project based on:
Klebsiella pneumoniae ATCC 4352
 NCBI: GCA_010374885
@ref Trait Model Prediction Confidence in % In training data
125439 spore_formation BacteriaNetⓘ no 97.90 no
125439 motility BacteriaNetⓘ yes 76.00 no
125439 gram_stain BacteriaNetⓘ negative 99.20 no
125439 oxygen_tolerance BacteriaNetⓘ aerobe 91.50 no
Predictions from Diaspora project based on:
Klebsiella pneumoniae ATCC 4352
NCBI: GCA_010374885.1
@ref Trait Model Prediction Confidence in % In training data
125438 gram-positive gram-positiveⓘ no 97.50 no
125438 anaerobic anaerobicⓘ no 97.68 yes
125438 aerobic aerobicⓘ no 58.72 no
125438 spore-forming spore-formingⓘ no 87.38 no
125438 thermophilic thermophileⓘ no 98.50 yes
125438 flagellated motile2+ⓘ no 77.34 no

External links

Literature

TitleAuthorsJournalDOIYear
Sustainable Antibacterial and Antiviral High-Performance Copper-Coated Filter Produced via Ion Beam Treatment.Jung S, Yang JY, Jang D, Kim T, Baek KH, Yoon H, Park JY, Kim SK, Hong J, Ryoo S, Jang HW, Lee SPolymers (Basel)10.3390/polym140510072022
Efficient Synthesis and Antibacterial Profile of Bis(2-hydroxynaphthalene- 1,4-dione).Novais JS, Rosandiski AC, de Carvalho CM, de Saules Silva LS, Dos S Velasco de Souza LC, Santana MV, Martins NRC, Castro HC, Ferreira VF, Gonzaga DTG, de Resende GO, de C da Silva FCurr Top Med Chem10.2174/15680266196661912101603422020
Antibacterial naphthoquinone derivatives targeting resistant strain Gram-negative bacteria in biofilms.Novais JS, Moreira CS, Silva ACJA, Loureiro RS, Sa Figueiredo AM, Ferreira VF, Castro HC, da Rocha DRMicrob Pathog10.1016/j.micpath.2018.03.0242018
Effects and time-kill assessment of amoxicillin used in combination with chloramphenicol against bacteria of clinical importance.Olajuyigbe OO, Coopoosamy RM, Afolayan AJActa Biochim Pol10.18388/abp.2016_14952017
Antibacterial Activities of Pyrenylated Coumarins from the Roots of Prangos hulusii.Tan N, Yazici-Tutunis S, Bilgin M, Tan E, Miski MMolecules10.3390/molecules220710982017
Klebsiella pneumoniae Planktonic and Biofilm Reduction by Different Plant Extracts: In Vitro Study.Paula-Ramos L, da Rocha Santos CE, Camargo Reis Mello D, Nishiama Theodoro L, De Oliveira FE, Back Brito GN, Junqueira JC, Jorge AOC, de Oliveira LDScientificWorldJournal10.1155/2016/35214132016
Searching for a potential antibacterial lead structure against bacterial biofilms among new naphthoquinone compounds.Moreira CS, Silva AC, Novais JS, Sa Figueiredo AM, Ferreira VF, da Rocha DR, Castro HCJ Appl Microbiol10.1111/jam.133692017
[Use of the materials based on partially reduced graphene-oxide with silver nanoparticle as bacteriostatic and bactericidal agent].Kedziora A, Gerasymchuk Y, Sroka E, Bugla-Ploskonska G, Doroszkiewicz W, Rybak Z, Hreniak DC, Wilgusz R, Strek WAPolim Med2013
Activity of the antiseptic polyhexanide against gram-negative bacteria.Fabry WH, Kock HJ, Vahlensieck WMicrob Drug Resist10.1089/mdr.2013.01132013
Hydroxyapatites and europium(III) doped hydroxyapatites as a carrier of silver nanoparticles and their antimicrobial activity.Wiglusz RJ, Kedziora A, Lukowiak A, Doroszkiewicz W, Strek WJ Biomed Nanotechnol10.1166/jbn.2012.14242012
Synthesis, Characterization and Antimicrobial Activity of d Metal Complexes of some 2-Substituted-1H-Benzimidazoles.Ulkuseven B, Tavman A, Otuk GMet Based Drugs10.1155/MBD.1999.1631999
Synthesis and evaluation of antimicrobial and anticonvulsant activities of some new 3-[2- (5-aryl-1,3,4-oxadiazol-2-yl/4-carbethoxymethylthiazol-2-yl) imino-4-thiazolidinon-5-ylidene]-5-substituted/nonsubstituted 1H-indole-2-ones and investigation of their structure-activity relationships.Altintas H, Ates O, Uyde-Dogan BS, Alp FI, Kaleli D, Ozdemir O, Birteksoz S, Otuk G, Atana D, Uzun MArzneimittelforschung2006
Synthesis and antimicrobial activity of some novel derivatives of benzofuran: part 1. Synthesis and antimicrobial activity of (benzofuran-2-yl)(3-phenyl-3-methylcyclobutyl) ketoxime derivatives.Koca M, Servi S, Kirilmis C, Ahmedzade M, Kazaz C, Ozbek B, Otuk GEur J Med Chem10.1016/j.ejmech.2005.07.0042005
Synthesis of some new 6-methylimidazo[2,1-b]thiazole-5-carbohydrazide derivatives and their antimicrobial activities.Ur F, Cesur N, Birteksoz S, Otuk GArzneimittelforschung10.1055/s-0031-12969472004
Synthesis and antimicrobial activity of [2-[2-(N, N-disubstituted thiocarbamoyl-sulfanyl)-acylamino] thiazol-4-yl]acetic acid ethyl esters.Ates O, Gursoy A, Altintas H, Otuk G, Birteksoz SArch Pharm (Weinheim)10.1002/ardp.2003900022003
Synthesis and evaluation of antibacterial activity of some 2-[[alpha-(4-substituted benzoyloxy)-alpha-phenylacetyl or methylacetyl]amino]-5-(4-methoxyphenyl)- 1,3,4-oxadiazoles.Kocabalkanli A, Ates O, Otuk GFarmaco10.1016/s0014-827x(01)01158-22001
Synthesis and antimicrobial activity of some 1,2,4-triazole-3-mercaptoacetic acid derivatives.Ulusoy N, Gursoy A, Otuk GFarmaco10.1016/s0014-827x(01)01128-42001
Synthesis of Mannich bases of some 2,5-disubstituted 4-thiazolidinones and evaluation of their antimicrobial activities.Kocabalkanli A, Ates O, Otuk GArch Pharm (Weinheim)10.1002/1521-4184(200102)334:2<35::aid-ardp35>3.0.co;2-42001
Synthesis and antimicrobial activity of 4-carbethoxymethyl-2-[(alpha-haloacyl)amino] thiazoles and 5-nonsubstituted/substituted 2-[(4-carbethoxymethylthiazol-2-yl)imino]-4-thiazolidinones.Ates O, Altintas H, Otuk GArzneimittelforschung10.1055/s-0031-13002512000
Corrected identification of a test organism (ATCC 4352) previously thought to be Escherichia coli.Brown CP, Wilson FHAppl Microbiol10.1128/am.23.3.661-661.19721972
@refStraininfoCulture collections
74465125696LMG 3128, ATCC 4352, DSM 789, IAM 12015, NCIB 10341, CIP 104216, NCIMB 10341, IFO 13277, KCTC 2690, NBRC 13277, BCRC 16082, CCRC 16082, CBMAI 699, JCM 20348, CNCTC 6120, CECT 8453, CCUG 58866

Culture collection no.

Culture collection no.
CCUG 58866
DSM 789
ATCC 4352
NCIB 10341
WDCM 00192
NCIMB 10341
NBRC 13277
IFO 13277
IAM 12015
CIP 104216
JCM 20348
CECT 8453
NCTC 13635

References

#447 Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 789
#20215 Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#41831 ; Curators of the CIP;
#61767 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 58866
#66792 Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368 Automatically annotated from API 20E .
#68374 Automatically annotated from API ID32E .
#68382 Automatically annotated from API zym .
#74465 Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID125696.1 )
#121885 Collection of Institut Pasteur ; Curators of the CIP; CIP 104216
#124043 Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438 Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439 Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

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