Haliangium ochraceum (DSM 14365, JCM 11303, AJ 13395)

Multimedia

• @ref:	66793
  manual_annotation:	1
  multimedia content:	EM_DSM_14365_1.jpg
  multimedia.multimedia content:	EM_DSM_14365_1.jpg
  caption:	electron microscopic image
  intellectual property rights:	© HZI/Manfred Rohde

Culture medium

@ref	Name	Growth	Medium link	Composition
5321	VY/4-SWS AGAR (DSMZ Medium 958)		Medium recipe at MediaDive	Name: VY/4-SWS AGAR (DSMZ Medium 958) Composition: NaCl 20.0 g/l Agar 15.0 g/l MgSO4 x 7 H2O 8.0 g/l Yeast cell paste 2.5 g/l CaCl2 x 2 H2O 1.0 g/l KCl 0.5 g/l NaHCO3 0.16 g/l KBr 0.08 g/l SrCl2 x 6 H2O 0.03 g/l Sodium beta-glycerophosphate 0.01 g/l Ferric citrate 0.01 g/l Cyanocobalamine 0.0005 g/l EDTA 0.0005 g/l FeSO4 x 7 H2O 0.0002 g/l H3BO3 3e-05 g/l CoCl2 x 6 H2O 2e-05 g/l ZnSO4 x 7 H2O 1e-05 g/l Na2MoO4 x 2 H2O 3e-06 g/l MnCl2 x 4 H2O 3e-06 g/l NiCl2 x 6 H2O 2e-06 g/l CuCl2 x 2 H2O 1e-06 g/l Distilled water

Culture temp

Observation

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions	external links
66794	enterobactin biosynthesis	100	3 of 3
66794	glycine betaine biosynthesis	100	5 of 5
66794	methylglyoxal degradation	100	5 of 5
66794	vitamin K metabolism	100	5 of 5
66794	glycogen metabolism	100	5 of 5
66794	reductive acetyl coenzyme A pathway	100	7 of 7
66794	factor 420 biosynthesis	100	5 of 5
66794	Entner Doudoroff pathway	100	10 of 10
66794	kanosamine biosynthesis II	100	2 of 2
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	cardiolipin biosynthesis	100	7 of 7
66794	starch degradation	100	10 of 10
66794	palmitate biosynthesis	100	22 of 22
66794	threonine metabolism	100	10 of 10
66794	biotin biosynthesis	100	4 of 4
66794	ethanol fermentation	100	2 of 2
66794	formaldehyde oxidation	100	3 of 3
66794	adipate degradation	100	2 of 2
66794	ppGpp biosynthesis	100	4 of 4
66794	suberin monomers biosynthesis	100	2 of 2
66794	taurine degradation	100	1 of 1
66794	coenzyme A metabolism	100	4 of 4
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	cellulose degradation	100	5 of 5
66794	folate polyglutamylation	100	1 of 1
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	sulfopterin metabolism	100	4 of 4
66794	gluconeogenesis	100	8 of 8
66794	C4 and CAM-carbon fixation	100	8 of 8
66794	phenylalanine metabolism	92.31	12 of 13
66794	leucine metabolism	92.31	12 of 13
66794	molybdenum cofactor biosynthesis	88.89	8 of 9
66794	CO2 fixation in Crenarchaeota	88.89	8 of 9
66794	valine metabolism	88.89	8 of 9
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	serine metabolism	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	NAD metabolism	88.89	16 of 18
66794	isoleucine metabolism	87.5	7 of 8
66794	photosynthesis	85.71	12 of 14
66794	glutamate and glutamine metabolism	85.71	24 of 28
66794	tetrahydrofolate metabolism	85.71	12 of 14
66794	degradation of aromatic, nitrogen containing compounds	83.33	10 of 12
66794	alanine metabolism	82.76	24 of 29
66794	proline metabolism	81.82	9 of 11
66794	propionate fermentation	80	8 of 10
66794	peptidoglycan biosynthesis	80	12 of 15
66794	phenylacetate degradation (aerobic)	80	4 of 5
66794	metabolism of amino sugars and derivatives	80	4 of 5
66794	citric acid cycle	78.57	11 of 14
66794	glutathione metabolism	78.57	11 of 14
66794	tyrosine metabolism	78.57	11 of 14
66794	heme metabolism	78.57	11 of 14
66794	vitamin B1 metabolism	76.92	10 of 13
66794	methionine metabolism	76.92	20 of 26
66794	ketogluconate metabolism	75	6 of 8
66794	acetate fermentation	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	cyclohexanol degradation	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	CMP-KDO biosynthesis	75	3 of 4
66794	purine metabolism	74.47	70 of 94
66794	flavin biosynthesis	73.33	11 of 15
66794	metabolism of disaccharids	72.73	8 of 11
66794	pentose phosphate pathway	72.73	8 of 11
66794	histidine metabolism	72.41	21 of 29
66794	benzoyl-CoA degradation	71.43	5 of 7
66794	ubiquinone biosynthesis	71.43	5 of 7
66794	pyrimidine metabolism	71.11	32 of 45
66794	arginine metabolism	70.83	17 of 24
66794	glycolysis	70.59	12 of 17
66794	cyanate degradation	66.67	2 of 3
66794	nitrate assimilation	66.67	6 of 9
66794	octane oxidation	66.67	2 of 3
66794	d-mannose degradation	66.67	6 of 9
66794	arachidonic acid metabolism	66.67	12 of 18
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	IAA biosynthesis	66.67	2 of 3
66794	selenocysteine biosynthesis	66.67	4 of 6
66794	acetoin degradation	66.67	2 of 3
66794	tryptophan metabolism	65.79	25 of 38
66794	polyamine pathway	65.22	15 of 23
66794	lysine metabolism	64.29	27 of 42
66794	vitamin B6 metabolism	63.64	7 of 11
66794	non-pathway related	63.16	24 of 38
66794	degradation of sugar alcohols	62.5	10 of 16
66794	dTDPLrhamnose biosynthesis	62.5	5 of 8
66794	urea cycle	61.54	8 of 13
66794	cysteine metabolism	61.11	11 of 18
66794	oxidative phosphorylation	60.44	55 of 91
66794	carotenoid biosynthesis	59.09	13 of 22
66794	androgen and estrogen metabolism	56.25	9 of 16
66794	lipid A biosynthesis	55.56	5 of 9
66794	lipid metabolism	54.84	17 of 31
66794	d-xylose degradation	54.55	6 of 11
66794	3-phenylpropionate degradation	53.33	8 of 15
66794	ribulose monophosphate pathway	50	1 of 2
66794	carnitine metabolism	50	4 of 8
66794	4-hydroxyphenylacetate degradation	50	5 of 10
66794	coenzyme M biosynthesis	50	5 of 10
66794	lactate fermentation	50	2 of 4
66794	glycolate and glyoxylate degradation	50	3 of 6
66794	toluene degradation	50	2 of 4
66794	pantothenate biosynthesis	50	3 of 6
66794	phenylmercury acetate degradation	50	1 of 2
66794	glycine metabolism	50	5 of 10
66794	butanoate fermentation	50	2 of 4
66794	vitamin E metabolism	50	2 of 4
66794	bile acid biosynthesis, neutral pathway	47.06	8 of 17
66794	sulfate reduction	46.15	6 of 13
66794	phenol degradation	45	9 of 20
66794	4-hydroxymandelate degradation	44.44	4 of 9
66794	mevalonate metabolism	42.86	3 of 7
66794	propanol degradation	42.86	3 of 7
66794	isoprenoid biosynthesis	42.31	11 of 26
66794	D-cycloserine biosynthesis	40	2 of 5
66794	3-chlorocatechol degradation	40	2 of 5
66794	lipoate biosynthesis	40	2 of 5
66794	degradation of sugar acids	40	10 of 25
66794	arachidonate biosynthesis	40	2 of 5
66794	gallate degradation	40	2 of 5
66794	bacilysin biosynthesis	40	2 of 5
66794	ethylmalonyl-CoA pathway	40	2 of 5
66794	myo-inositol biosynthesis	40	4 of 10
66794	degradation of pentoses	39.29	11 of 28
66794	degradation of hexoses	38.89	7 of 18
66794	phosphatidylethanolamine bioynthesis	38.46	5 of 13
66794	phenylpropanoid biosynthesis	38.46	5 of 13
66794	ascorbate metabolism	36.36	8 of 22
66794	cholesterol biosynthesis	36.36	4 of 11
66794	allantoin degradation	33.33	3 of 9
66794	sulfoquinovose degradation	33.33	1 of 3
66794	sphingosine metabolism	33.33	2 of 6
66794	methane metabolism	33.33	1 of 3
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	aclacinomycin biosynthesis	28.57	2 of 7
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	catecholamine biosynthesis	25	1 of 4
66794	alginate biosynthesis	25	1 of 4
66794	chlorophyll metabolism	22.22	4 of 18
66794	vitamin B12 metabolism	20.59	7 of 34

Compound production

Oxygen tolerance

Isolation source categories

Isolation

Taxonmaps

69479

Global distribution of 16S sequence AB016470 (>99% sequence identity) for Haliangium ochraceum subclade from Microbeatlas

Aquatic Samples	560
Soil Samples	336
Animal Samples	18
Plant Samples	67
Total Samples	981

Risk assessment

GC content

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
67770	Haliangium ochraceum gene for 16S rRNA, partial sequence	AB016470	1507		502025

Genome sequences

@ref	Description	Accession	Assembly level	Database	NCBI tax ID
66792	Haliangium ochraceum DSM 14365	GCA_000024805	complete		502025
66792	Haliangium ochraceum DSM 14365	502025.10	complete	PATRIC	502025
66792	Haliangium ochraceum SMP-2, DSM 14365	646311933	complete	JGI IMG/M	502025

Predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Analysis of the Genome and Metabolome of Marine Myxobacteria Reveals High Potential for Biosynthesis of Novel Specialized Metabolites.	Amiri Moghaddam J, Crusemann M, Alanjary M, Harms H, Davila-Cespedes A, Blom J, Poehlein A, Ziemert N, Konig GM, Schaberle TF	Sci Rep	10.1038/s41598-018-34954-y	2018
Biotechnology	Heterologous Production of the Marine Myxobacterial Antibiotic Haliangicin and Its Unnatural Analogues Generated by Engineering of the Biochemical Pathway.	Sun Y, Feng Z, Tomura T, Suzuki A, Miyano S, Tsuge T, Mori H, Suh JW, Iizuka T, Fudou R, Ojika M	Sci Rep	10.1038/srep22091	2016
Metabolism	Isolation and Biosynthetic Analysis of Haliamide, a New PKS-NRPS Hybrid Metabolite from the Marine Myxobacterium Haliangium ochraceum.	Sun Y, Tomura T, Sato J, Iizuka T, Fudou R, Ojika M	Molecules	10.3390/molecules21010059	2016
Phylogeny	A novel family VII esterase with industrial potential from compost metagenomic library.	Kang CH, Oh KH, Lee MH, Oh TK, Kim BH, Yoon J	Microb Cell Fact	10.1186/1475-2859-10-41	2011
Genetics	Investigation of cytochromes P450 in myxobacteria: excavation of cytochromes P450 from the genome of Sorangium cellulosum So ce56.	Khatri Y, Hannemann F, Perlova O, Muller R, Bernhardt R	FEBS Lett	10.1016/j.febslet.2011.04.035	2011
Genetics	Complete genome sequence of Haliangium ochraceum type strain (SMP-2).	Ivanova N, Daum C, Lang E, Abt B, Kopitz M, Saunders E, Lapidus A, Lucas S, Glavina Del Rio T, Nolan M, Tice H, Copeland A, Cheng JF, Chen F, Bruce D, Goodwin L, Pitluck S, Mavromatis K, Pati A, Mikhailova N, Chen A, Palaniappan K, Land M, Hauser L, Chang YJ, Jeffries CD, Detter JC, Brettin T, Rohde M, Goker M, Bristow J, Markowitz V, Eisen JA, Hugenholtz P, Kyrpides NC, Klenk HP	Stand Genomic Sci	10.4056/sigs.69.1277	2010
Cultivation	Characteristics and living patterns of marine myxobacterial isolates.	Zhang YQ, Li YZ, Wang B, Wu ZH, Zhang CY, Gong X, Qiu ZJ, Zhang Y	Appl Environ Microbiol	10.1128/AEM.71.6.3331-3336.2005	2005
Phylogeny	Haliangium ochraceum gen. nov., sp. nov. and Haliangium tepidum sp. nov.: novel moderately halophilic myxobacteria isolated from coastal saline environments.	Fudou R, Jojima Y, Iizuka T, Yamanaka S	J Gen Appl Microbiol	10.2323/jgam.48.109	2002

Straininfo Link

@ref	Straininfo	Culture collections
75349	100667	DSM 14365, JCM 11303, CIP 107738

Culture collection no.