Cite this strain

"Methanosaeta pelagica" (DSM 24271, NBRC 105920, 03d30q)

BacDive ID 7052

Type strain

Strain Designation 03d30q

Culture col. no. DSM 24271 NBRC 105920 03d30q

NCBI tax ID(s) 1110545

Special Collections DSMZ

Links

Name and taxonomic classification

"Methanosaeta pelagica" 03d30q is an anaerobe, mesophilic archaeon that was isolated from tidal flat sediment.

anaerobe mesophilic 16S sequence Archaea

Culture Collection Numbers

DSM 24271 NBRC 105920 03d30q

Last LPSN update 2024-12-09 10:43:54

Domain Archaea

Phylum Methanobacteriota

Class Methanosarcinia

Order Methanosarcinales

Family Methanotrichaceae

Genus Methanosaeta

Species "Methanosaeta pelagica"

Full scientific name Methanosaeta pelagica Mori et al. 2012

Morphology

Colony morphology

17601

Incubation period>14 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
17601	METHANOSAETA PELAGICA MEDIUM (DSMZ Medium 1329)		Medium recipe at MediaDive	Name: METHANOSAETA PELAGICA MEDIUM (DSMZ Medium 1329) Composition: Na-acetate 6.54038 g/l MgCl2 x 6 H2O 3.04088 g/l Yeast extract 1.49551 g/l Na2CO3 1.49551 g/l KH2PO4 1.18644 g/l NH4Cl 0.538385 g/l Trypticase peptone 0.398804 g/l Na2S x 9 H2O 0.358923 g/l K2HPO4 0.209372 g/l 2-Mercaptoethanesulfonic acid 0.139581 g/l Nitrilotriacetic acid 0.0255234 g/l FeCl2 x 4 H2O 0.00199402 g/l NaCl 0.00199402 g/l Sodium resazurin 0.000498504 g/l CaCl2 x 2 H2O 0.000199402 g/l MnCl2 x 4 H2O 0.000199402 g/l NiCl2 x 6 H2O 0.000199402 g/l ZnCl2 0.000199402 g/l Pyridoxine hydrochloride 9.97009e-05 g/l Na2WO4 x 2 H2O 7.97607e-05 g/l CoCl2 x 6 H2O 5.98205e-05 g/l Na2MoO4 x 2 H2O 5.98205e-05 g/l Na2SeO3 x 5 H2O 5.98205e-05 g/l Thiamine HCl 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l CuCl2 3.98804e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l H3BO3 1.99402e-05 g/l Vitamin B12 9.97009e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
17601	positive	growth	30	mesophilic

Physiology and metabolism

Oxygen tolerance

17601

Oxygen toleranceanaerobe

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Tidal flat
#Environmental	#Terrestrial	#Sediment

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
17601	tidal flat sediment	Chiba Prefecture, Tokyo Bay	Japan	JPN	Asia

Taxonmaps

69479

Global distribution of 16S sequence AB679167 (>99% sequence identity) for Methanosaeta pelagica subclade from Microbeatlas

Aquatic Samples	37
Soil Samples	1
Animal Samples
Plant Samples
Total Samples	38

Safety information

Sequence information

GC content

@ref	GC-content (mol%)	Method
17601	45.4	high performance liquid chromatography (HPLC)

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
17601	Methanosaeta pelagica gene for 16S rRNA, partial sequence	AB679167	1383		1110545

Genome-based predictions

External links

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Aceticlastic and NaCl-requiring methanogen "Methanosaeta pelagica" sp. nov., isolated from marine tidal flat sediment.	Mori K, Iino T, Suzuki K, Yamaguchi K, Kamagata Y	Appl Environ Microbiol	10.1128/AEM.07484-11	2012

Culture collection no.

Culture collection no.
DSM 24271
NBRC 105920
03d30q

References

#17601	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 24271
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

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