Cite this strain

Mycobacterium intermedium (DSM 44049, ATCC 51848, CIP 104542)

Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 8426

Type strain

Strain Designation 1669/91

Culture col. no. DSM 44049 ATCC 51848 CIP 104542 CCUG 37583 JCM 13572 1 more

NCBI tax ID(s) 28445

Special Collections DSMZ CCUG JCM CIP

Links

Name and taxonomic classification

Mycobacterium intermedium 1669/91 is an obligate aerobe, mesophilic, rod-shaped bacterium that was isolated from sputum of a patient with pulmonary disease.

rod-shaped obligate aerobe mesophilic genome sequence 16S sequence Bacteria

SI-ID 43212

ATCC 51848, DSM 44049, CCUG 37583, CIP 104542, JCM 13572

Last LPSN update 2024-12-09 10:44:03

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Mycobacteriales

Family Mycobacteriaceae

Genus Mycobacterium

Species Mycobacterium intermedium

Full scientific name Mycobacterium intermedium Meier et al. 1993

Morphology

Cell morphology

@ref	Cell shape	Motility
120149	rod-shaped
125439

Multimedia

@ref:	11463
manual_annotation:	1
multimedia content:	DSM_44049.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_44049.jpg
caption:	Medium 645 37°C
intellectual property rights:	© Leibniz-Institut DSMZ

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
11463	MIDDLEBROOK MEDIUM (DSMZ Medium 645)	Medium recipe at MediaDive	Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
11463	LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354)	Medium recipe at MediaDive	Name: LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) Composition: Potato flour 18.6104 g/l L-Asparagin 2.23325 g/l KH2PO4 1.55087 g/l Mg-citrate 0.372208 g/l Malachite green 0.248139 g/l MgSO4 0.148883 g/l Glycerol Fresh egg mixture Distilled water
42285	MEDIUM 55 - for Mycobacterium
120149	CIP Medium 55	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
11463	positive	growth	37	mesophilic
42285	positive	growth	37	mesophilic
67770	positive	growth	37	mesophilic
120149	positive	growth	37-45
120149	negative	growth	10

Physiology and metabolism

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
120149	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
120149	amylase	-
68382	beta-galactosidase	-	3.2.1.23	from API zym
120149	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
120149	caseinase	-	3.4.21.50
120149	catalase	+	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
120149	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
120149	gelatinase	-
120149	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	+		from API zym
120149	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
120149	ornithine decarboxylase	-	4.1.1.17
120149	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
120149	tween esterase	+
120149	urease	-	3.5.1.5
68382	valine arylamidase	+		from API zym

Metabolite production

@ref	Chebi-ID	Metabolite	Production
120149	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
120149	15688 ChEBI	acetoin	-

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
120149	16947 ChEBI	citrate	-	carbon source
120149	4853 ChEBI	esculin	-	hydrolysis
120149	17632 ChEBI	nitrate	-	reduction
120149	17632 ChEBI	nitrate	-	respiration
120149	16301 ChEBI	nitrite	-	reduction

Oxygen tolerance

@ref	Oxygen tolerance
120149	obligate aerobe
125439	obligate aerobe

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
120149	-	+	+	+	+	+	+	+	-	-	+	-	-	-	-	-	+	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Infection	#Inflammation
#Infection	#Patient
#Host Body-Site	#Oral cavity and airways
#Host Body Product	#Fluids

Isolation

@ref	Sample type
11463	sputum of a patient with pulmonary disease
53748	Human sputum
67770	Sputum from a patient with pulmonary disease
120149	Human, Sputum

Taxonmaps

69479

Global distribution of 16S sequence X67847 (>99% sequence identity) for Mycobacterium intermedium from Microbeatlas

Aquatic Samples	1793
Soil Samples	988
Animal Samples	242
Plant Samples	243
Total Samples	3266

Safety information

Risk assessment

@ref	Biosafety level	Biosafety level comment
11463	2	Risk group (German classification) According to TRBA 466
120149	1	Risk group (French classification) According to TRBA 466

Sequence information

GC content

@ref	GC-content (mol%)	Method
67770	65.8	genome sequence analysis

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	M.intermedium DNA for 16S rRNA	X67847	1441	28445
20218	Mycobacterium intermedium strain CIP 104542 16S ribosomal RNA gene, partial sequence	AF547938	543	28445
20218	Mycobacterium intermedium strain DSM 44049 16S-23S ribosomal RNA intergenic spacer, partial sequence	HM022204	277	28445

Genome sequences

@ref	Description	Accession	Assembly level	Database	NCBI tax ID
66792	Mycobacterium intermedium strain DSM 44049	28445.6	wgs	PATRIC	28445
67770	Mycobacterium intermedium DSM 44049	GCA_002086275	contig		28445
66792	Mycobacterium intermedium DSM 44049	GCA_025822065	scaffold		28445

Genome-based predictions

Predictions

Predictions from Diaspora project based on:
Mycobacterium intermedium DSM 44049
NCBI: GCA_025822065

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	59.40	no
125439	motility	BacteriaNetⓘ	no	84.80	no
125439	gram_stain	BacteriaNetⓘ	positive	91.60	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	96.10	no

Predictions from Diaspora project based on:
Mycobacterium intermedium strain DSM 44049
PATRIC: 28445.6

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	89.07	no
125438	anaerobic	anaerobicⓘ	no	97.73	no
125438	spore-forming	spore-formingⓘ	no	59.79	no
125438	aerobic	aerobicⓘ	yes	79.92	no
125438	thermophilic	thermophileⓘ	no	96.40	yes
125438	flagellated	motile2+ⓘ	no	88.50	no

External links

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Mycobacterium intermedium sp. nov.	Meier A, Kirschner P, Schroder KH, Wolters J, Kroppenstedt RM, Bottger EC	Int J Syst Bacteriol	10.1099/00207713-43-2-204	1993

Straininfo Link

@ref	Straininfo	Culture collections
77825	43212	ATCC 51848, DSM 44049, CCUG 37583, CIP 104542, JCM 13572

Culture collection no.

Culture collection no.
DSM 44049
ATCC 51848
CIP 104542
CCUG 37583
JCM 13572
1669/91

References

#11463	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44049
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#42285	; Curators of the CIP;
#53748	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 37583
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#77825	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID43212.1 )
#120149	Collection of Institut Pasteur ; Curators of the CIP; CIP 104542
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

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